In an article, author is Zheng, Ke-Wei, once mentioned the application of 181124-40-3, Name is Benzo[d]thiazole-6-sulfonyl chloride, molecular formula is C7H4ClNO2S2, molecular weight is 233.7, MDL number is MFCD03086100, category is thiazoles. Now introduce a scientific discovery about this category, COA of Formula: C7H4ClNO2S2.
Detection of genomic G-quadruplexes in living cells using a small artificial protein
G-quadruplex (G4) structures formed by guanine-rich nucleic acids are implicated in essential physiological and pathological processes and serve as important drug targets. The genome-wide detection of G4s in living cells is important for exploring the functional role of G4s but has not yet been achieved due to the lack of a suitable G4 probe. Here we report an artificial 6.7 kDa G4 probe (G4P) protein that binds G4s with high affinity and specificity. We used it to capture G4s in living human, mouse, and chicken cells with the ChIP-Seq technique, yielding genome-wide landscape as well as details on the positions, frequencies, and sequence identities of G4 formation in these cells. Our results indicate that transcription is accompanied by a robust formation of G4s in genes. In human cells, we detected up to >123 000 G4P peaks, of which >1/3 had a fold increase of >= 5 and were present in >60% promoters and similar to 70% genes. Being much smaller than a scFv antibody (27 kDa) or even a nanobody (12-15 kDa), we expect that the G4P may find diverse applications in biology, medicine, and molecular devices as a G4 affinity agent.
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Reference:
Thiazole | C3H3NS – PubChem,
,Thiazole | chemical compound | Britannica