Stoneman, Michael R.; Raicu, Valerica published the artcile< Dielectric spectroscopy based detection of specific and nonspecific cellular mechanisms>, SDS of cas: 2591-17-5, the main research area is dielec spectroscopy GPCR ligand interaction intracellular transport; G protein-coupled receptor; GPCR; dielectric relaxation; dielectric spectroscopy; label-free detection; ligand binding.
Using radiofrequency dielec. spectroscopy, we have investigated the impact of the interaction between a G protein-coupled receptor (GPCR), the sterile2 α-factor receptor protein (Ste2), and its cognate agonist ligand, the α-factor pheromone, on the dielec. properties of the plasma membrane in living yeast cells (Saccharomyces cerevisiae). The dielec. properties of a cell suspension containing a saturating concentration of α-factor were measured over the frequency range 40 Hz-110 MHz and compared to the behavior of a similarly prepared suspension of cells in the absence of α-factor. A spherical three-shell model was used to determine the elec. phase parameters for the yeast cells in both types of suspensions. The relative permittivity of the plasma membrane showed a significant increase after exposure to α-factor (by 0.06 ± 0.05). The equivalent experiment performed on yeast cells lacking the ability to express Ste2 showed no change in plasma membrane permittivity. Interestingly, a large change also occurred to the elec. properties of the cellular interior after the addition of α-factor to the cell suspending medium, whether or not the cells were expressing Ste2. We present a number of different complementary experiments performed on the yeast to support these dielec. data and interpret the results in terms of specific cellular reactions to the presence of α-factor.
Sensors published new progress about Cell membrane. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, SDS of cas: 2591-17-5.
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica