Luo, Jinqiu; Yang, Jinfeng; Li, Guangjie; Yang, Sheng; Zhou, Yibo; Li, Jun-Bin; Huang, Ge; Hu, Yibo; Zou, Shuangfa; Zeng, Qinghai; Yang, Ronghua published the artcile< Noncovalently Caged Firefly Luciferins Enable Amplifiable Bioluminescence Sensing of Hyaluronidase-1 Activity in Vivo>, Product Details of C11H8N2O3S2, the main research area is noncovalently caged firefly luciferins bioluminescence sensor hyaluronidase 1; bioluminescence; enzymatic assay; hyaluronidase; in vivo bioimaging; nanosensor; signal amplification.
Hyaluronidase 1 (Hyal-1) is an important enzyme involved in intracellular hyaluronic acid (HA) catabolism for performing various physiol. functions, and its aberrant level is closely associated with many malignant diseases. Bioluminescence imaging is advantageous for monitoring Hyal-1 activity in vivo, but it remains challenging to design an available probe for differentiating Hyal-1 from other isoforms by a traditional strategy that covalently masks the firefly luciferase substrate. Herein, we, for the first time, present a noncovalently caging approach to construct a Hyal-1-specific bioluminogenic nanosensor by entrapping D-luciferin (D-Luc) inside the cholesterylamine-modified HA (CHA) nanoassembly to inhibit the bioluminescence production When encountered with intracellular Hyal-1, CHA could be fully dissembled to liberate multiple copies of the loaded D-Luc, thereby emitting light by the luciferase-catalyzed bioluminescence reaction. Because of its cascade signal amplification feature, D-Luc@CHA displayed a remarkable “”turn-on”” response (248-fold) to 5μg/mL Hyal-1 with a detection limit of 0.07 ng/mL. Importantly, bioluminescence imaging results validated that D-Luc@CHA could be competent for dynamically visualizing endogenous Hyal-1 changes in living cells and animals and possessed the capability of discriminating between normal and cancer cells, thus offering a promising toolbox to evaluate Hyal-1 roles in biol. processes as well as to diagnose Hyal-1-related diseases.
ACS Sensors published new progress about Bioluminescent imaging. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Product Details of C11H8N2O3S2.
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica