Liu, Pei-Han; Urban, Pawel L. published the artcile< Spontaneous luminescence color change in the firefly luciferase assay system>, Application of C11H7KN2O3S2, the main research area is luminescence color firefly luciferase; ATP assay; Color switching; Luciferase; Luminescence.
The temporal effects of luciferase reaction luminescence have only been discussed in the context of light intensity (flash vs. glow). However, alterations in the color of the light emitted over the luciferase reaction have not been reported. Here, the authors show a temporal change in the light color emitted during the reaction catalyzed by unmodified firefly luciferase when concentrations of one of the substrates, ATP, are gradually increased. The temporal color change from green to red occurs within the first few minutes of the luciferase reaction when an ATP-containing solution is either added or synthesized in situ with the aid of an autocatalytic reaction occurring simultaneously. This color change is not accompanied by pH changes. An anal. of the red and green channels demonstrates dissimilar kinetics, suggesting the coexistence of two or more temporally shifted luminescence pathways. The implications of these findings might improve dual-color biosensing/imaging protocols and influence the engineering of biophotonic systems.
Analytical Biochemistry published new progress about Color. 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, Application of C11H7KN2O3S2.
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica