Category: thiazoleOn May 11, 2020 ,《Label-free colorimetric detection of acid phosphatase and screening of its inhibitors based on biomimetic oxidase activity of MnO2 nanosheets》 appeared in ACS Biomaterials Science & Engineering. The author of the article were Wang, Jing; Lu, Qiaoyun; Weng, Chenyuan; Li, Xiaoyun; Yan, Xiaoqiang; Yang, Wei; Li, Bingzhi; Zhou, Xuemin. The article conveys some information:
In this research, we attempted to develop a sensitive colorimetric sensing strategy for the detection of acid phosphatase (ACP) based on MnO2 nanosheets and explored its applications in screening and evaluating inhibitors of ACP. The MnO2 nanosheets exhibit intrinsic biomimetic oxidase activity, which can catalyze the oxidation of the colorless 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonate) diammonium salt (ABTS) into green oxidized ABTS (oxABTS). Upon the introduction of ACP, L-ascorbic acid-2-phosphate can be dephosphorylated to ascorbic acid, which arouses the disintegration of MnO2 nanosheets into Mn2+ ions. This disintegration weakens the enzyme mimicking activity of the MnO2 nanosheets, leading to the impediment of the oxidation of ABTS. Conversely, in the absence of ACP, the ABTS is rapidly oxidized by MnO2, leading to a significant colorimetric signal change. The absorbance difference at 420 nm displayed a linear relationship with the concentration of ACP ranging from 0.075 to 0.45 mU·mL-1, generating a detection limit of 0.046 mU·mL-1. In the inhibition assays, this sensing platform provided simple detection for parathion-Me (PM), a representative inhibitor of ACP. The facile evaluation of the inhibitory effect of PM, including its IC50 toward ACP, was also realized. The experimental part of the paper was very detailed, including the reaction process of ABTS Diammonium(cas: 30931-67-0Category: thiazole)
ABTS Diammonium(cas: 30931-67-0) belongs to anime. Milder oxidation, using reagents such as NaOCl, can remove four hydrogen atoms from primary amines of the type RCH2NH2 to form nitriles (R―C≡N), and oxidation with reagents such as MnO2 can remove two hydrogen atoms from secondary amines (R2CH―NHR′) to form imines (R2C=NR′). Tertiary amines can be oxidized to enamines (R2C=CHNR2) by a variety of reagents.Category: thiazole
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica