Evans, Bridget L.; Hurlstone, Adam F. L.; Clayton, Peter E.; Stevens, Adam; Shiels, Holly A. published the artcile< Glucose uptake as an alternative to oxygen uptake for assessing metabolic rate in Danio rerio larvae>, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is glucose uptake metabolic rate Danio rerio larvae; Cardiorespiratory; Development; Glucose; Metabolism; Method comparison; Respirometry; Zebrafish larvae.
Respirometry, based on oxygen uptake, is commonly employed for measuring metabolic rate. There is a growing need for metabolic rate measurements suitable for developmental studies, particularly in Danio rerio, where many important developmental stages occur at < 4 mm. However, respirometry becomes more challenging as the size of the organism reduces. Addnl., respirometry can be costly and require significant experience and tech. knowledge which may prohibit uptake in non-specialist/non-physiol. laboratories Thus, using equipment routine in most developmental/mol. biol. laboratories, we measured glucose uptake in 96-h post fertilization (hpf) zebrafish larvae and compared it to stop-flow respirometry measures of oxygen uptake to test whether glucose uptake was a suitable alternative measure of metabolic rate. A Passing-Bablok regression revealed that within a 95% limit of agreement, the rate of glucose uptake and the rate of oxygen uptake were equivalent as measures of metabolic rate in 96 hpf Danio rerio larvae. Thus, the methodol. we outline here may be a useful alternative or a complementary method for assessing metabolic rate in small organisms. Current Research in Physiology published new progress about Danio rerio. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica