Morciano, Giampaolo; Imamura, Hiromi; Patergnani, Simone; Pedriali, Gaia; Giorgi, Carlotta; Pinton, Paolo published the artcile< Measurement of ATP concentrations in mitochondria of living cells using luminescence and fluorescence approaches>, Reference of 2591-17-5, the main research area is ATP; Bioluminescence; Biosensor; FRET; Fluorescence; Live cell imaging; Luciferase assay; Mitochondria.
Adenosine 5′-triphosphate (ATP) is the central metabolite in the energy metabolism of cells and is hydrolyzed to ADP and inorganic phosphate to provide free energy in various cellular processes. ATP also functions as an intracellular signaling mol. Thus, it is important to know the ATP concentration within cells to understand cellular activities. Here, we describe two methods to detect ATP concentrations in the cytoplasm and mitochondrial matrix using genetically encoded luminescent or fluorescent biosensors. These methods enable quant. investigation of ATP concentration dynamics in living cells, single cells and cell populations.
Methods in Cell Biology published new progress about 2591-17-5. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Reference of 2591-17-5.
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica