Moriguchi, Maiko; Takahashi, Ryo; Kang, Bubwoong; Kuse, Masaki published the artcile< Expression of recombinant apopholasin using a baculovirus-silkworm multigene expression system and activation via dehydrocoelenterazine>, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is expression recombinant apopholasin baculovirus silkworm multigene system activation dehydrocoelenterazine; Apopholasin; Baculovirus–silkworm multigene expression system; Chromophore; Dehydrocoelenterazine; Pholasin.
Pholasin is a photoprotein derived from the glowing bivalve mollusk, Pholas dactylus. Even though the chem. structure of the prosthetic group (chromophore) responsible for the light emission character of the mollusk remains unknown, research showed that the presence of dehydrocoelenterazine (DCL) increased light emission and that the dithiothreitol adduct of DCL was isolated from Pholasin. To date, the authors’ research has been focused on activating apopholasin, the naturally occurring apoprotein of Pholasin, using DCL. In the current study, the expression of recombinant apopholasin via a baculovirus-silkworm multigene expression system is reported. Addnl., the purification of apopholasin using a Flag-affinity column, the activation of apopholasin using DCL, and the initiation of its luminescent character through the addition of a peroxidase-hydrogen peroxide mixture are reported. The peroxidase-H2O2-dependent luminescence was observed from the recombinant apopholasin activated with DCL.
Bioorganic & Medicinal Chemistry Letters published new progress about Bioluminescence. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica