Verhoef, Lisette G. G. C.; Mattioli, Michela; Ricci, Fernanda; Li, Yao-Cheng; Wade, Mark published the artcile< Multiplex detection of protein-protein interactions using a next generation luciferase reporter>, Recommanded Product: Potassium (S)-2-(6-hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylate, the main research area is protein protein interactions assay luciferase reporter; Cancer; Interaction; Luciferase; MDM2; PPI; p53.
Cell-based assays of protein-protein interactions (PPIs) using split reporter proteins can be used to identify PPI agonists and antagonists. Split luciferase systems offer advantages over those that use split fluorescent proteins (FPs). This is since split luciferase offers a greater signal:noise ratio and, unlike split FPs, the PPI can be reversed upon small mol. treatment. While multiplexed PPI assays using luciferase have been reported, they suffer from low signal:noise and require fairly complex spectral deconvolution during anal. Furthermore, the luciferase enzymes used are large, which limits the range of PPIs that can be interrogated due to steric hindrance from the split luciferase fragments. Here, we report a multiplexed PPI assay based on split luciferases from Photinus pyralis (firefly luciferase, FLUC) and the deep-sea shrimp, Oplophorus gracilirostris (NanoLuc, NLUC). Specifically, we show that the binding of the p53 tumor suppressor to its two major neg. regulators, MDM2 and MDM4, can be simultaneously measured within the same sample, without the requirement for complex filters or deconvolution. We provide chem. and genetic validation of this system using MDM2-targeted small mols. and mutagenesis, resp. Combined with the superior signal:noise and smaller size of split NanoLuc, this multiplexed PPI assay format can be exploited to study the induction or disruption of pairwise interactions that are prominent in many cell signaling pathways.
Biochimica et Biophysica Acta, Molecular Cell Research published new progress about Bioassay. 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, Recommanded Product: Potassium (S)-2-(6-hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylate.
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica