Day: October 18, 2022

Fahmy, H. T. Y.; Bekhit, A. A. published the artcile< Synthesis of some new bis-thiazoles as possible anticancer agents>, Reference of 10574-69-3, the main research area is rhodanine preparation antitumor agent.

Several new 5-(2,3-dihydrothiazol-2-ylidenyl)rhodanines I (R1 = Ph; R2 = Ph, 4-ClC6H4, 4-MeC6H4; R3 = H) and 5-(4-oxothiazolidinon-2-ylidenyl)rhodanine II were synthesized through the reaction of 5-thiocarbamoyl rhodanine III [R1 = Ph; R4 = C(S)NHPh] with phenacyl bromides or chloroacetic acid, resp. The synthesis of arylidenes IV (R5 = H, Cl, MeO) was also described. The 5-(4-amino-5-cyano-2,3-dihydrothiazol-2-ylidenyl)rhodanines I (R1 = Ph, CH2Ph; R2 = NH2; R3 = CN) were obtained through reaction of rhodanines III (R1 = Ph, CH2Ph; R4 = H) with a thiazolium salt. All the prepared compounds were screened for their anticancer activity using the in vitro anticancer screening program of the NCI. Three compounds showed promising anticancer activity against particular human cell lines used in the assay.

Pharmazie published new progress about Antitumor agents. 10574-69-3 belongs to class thiazole, and the molecular formula is C10H9NOS2, Reference of 10574-69-3.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Wang, Fang I.; Ding, Gucci; Ng, Garmen S.; Dixon, S. Jeffrey; Chidiac, Peter published the artcile< Luciferase-based GloSensor cAMP assay: Temperature optimization and application to cell-based kinetic studies>, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is luciferase glosensor cAMP assay temperature optimization kinetics drug discovery; Drug discovery; GPCRs; GloSensor; High-throughput screening; Optimization.

G protein-coupled receptors (GPCRs) are an important receptor superfamily and common therapeutic targets. The second messenger cyclic adenosine monophosphate (cAMP) is a key mediator in many GPCR signaling pathways. Monitoring intracellular cAMP levels can help identify orthosteric agonists and antagonists, as well as allosteric modulators. In this regard, luminescence-based biosensors have revolutionized our ability to monitor GPCR signaling kinetics. The GloSensor cAMP assay enables real-time monitoring of signaling downstream of many GPCRs. However, it is crucial to optimize assay conditions such as temperature As well, it has not been reported whether the effects of temperature on biosensor activity are reversible. Here, we describe the temperature sensitivity and reversibility of the GloSensor cAMP assay, and which GloSensor version is optimal for measuring cytosolic cAMP. We also present a detailed protocol for monitoring cAMP levels in live cells expressing endogenous or exogenous GPCRs. Temperature optimization studies were carried out using HEK293H cells transiently transfected with the adenosine receptor A2a and the GloSensor plasmid (pGloSensor-20F or -22F). We found that preincubation and luminescence reading at room temperature were optimal as compared to higher temperatures As well, the GloSensor-22F biosensor had a superior signal-to-background ratio and the effect of temperature on biosensor activity was reversible. However, thermal instability of the biosensor may pose a problem for in vivo studies. Nevertheless, the GloSensor cAMP assay can be applied to analyze signaling by a wide range of GPCRs for drug discovery purposes.

Methods (Amsterdam, Netherlands) published new progress about Adenosine A2A receptors Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Singh, Kamaljit; Singh, Sarbjit; Taylor, John A. published the artcile< Monoazo disperse dyes-part 1: synthesis, spectroscopic studies and technical evaluation of monoazo disperse dyes derived from 2-aminothiazoles>, Application of C9H7N3O2S, the main research area is aminothiazole diazo aminoacetanilide coupling component disperse azo dye; polyester fabric disperse dyeing thiadiazole azo dye preparation.

Novel disperse dyes have been prepared from thiazolyl diazonium salts and coupling components based on m-aminoacetanilide derivatives Depending upon various substituents incorporated into the chromophore, absorption maxima varied from 495 to 591 nm in various organic solvents. These dyes were chromophorically strong as evidenced both by molar extinction coefficient in solvent and by strength and build-up on polyester.

Dyes and Pigments published new progress about Disperse azo dyes. 57493-24-0 belongs to class thiazole, and the molecular formula is C9H7N3O2S, Application of C9H7N3O2S.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica