Day: October 19, 2022

《Bioinformatic analysis of differentially expressed genes and identification of key genes in EBV-transformed lymphoblasts》 was written by Tang, Yunlian; Zhong, Yating; Fu, Ting; Zhang, Yang; Cheng, Ailan; Dai, Yongming; Qu, Jiani; Gan, Runliang. Quality Control of 6-Chlorobenzothiazol-2-ylamine And the article was included in Biomedicine & Pharmacotherapy on August 31 ,2019. The article conveys some information:

Although the Epstein-Barr virus (EBV) is a well-known human oncogenic virus, its mol. mechanisms involved in the transformation of healthy human cells remain poorly understood. In this study, human lymphocytes were isolated from the peripheral blood of healthy adults, and lymphocytes were transformed in vitro by EBV. Agilent human whole genome microarrays were used to detect the differential gene expression profiles of EBV-transformed lymphoblasts and healthy peripheral blood lymphocytes (PBLs). By constructing the gene functional network of EBV-induced lymphocyte transformation, we screened out candidate key genes in this process and verified their expression levels by real-time quant. polymerase chain reaction (RT-qPCR) and Western blot. In the EBV-transformed lymphoblasts, 2335 differentially expressed genes, including 1328 up-regulated and 1007 down-regulated, were screened out. Five candidate key genes, namely, PLK1, E2F1, PTPN11, BIRC5 and FYN were mainly screened out according to the results of LIMMA, String, Cytoscape software anal. RT-qPCR and Western blot showed that PLK1, E2F1, PTPN11, BIRC5 genes had increased expression levels, and FYN gene was down-regulated in EBV-transformed lymphoblasts. Silencing of PLK1 gene in Raji cells could inhibit cell proliferation and invasion, and induce cell cycle arrest and apoptosis. In conclusion, PLK1, E2F1, PTPN11, BIRC5 and FYN are the candidate key mols. of EBV-transformed lymphocytes. The experimental process involved the reaction of 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Quality Control of 6-Chlorobenzothiazol-2-ylamine)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Quality Control of 6-Chlorobenzothiazol-2-ylamineThiazoles can also be used as protected formyl groups, which can be released in later stages of complex natural product synthesis.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

《I2/DMSO mediated multicomponent reaction for the synthesis of 2-arylbenzo[d]imidazo[2,1-b]thiazole derivatives》 was published in Organic & Biomolecular Chemistry in 2019. These research results belong to Jana, Asim; Bhaumick, Prabhas; Panday, Anoop Kumar; Mishra, Richa; Choudhury, Lokman H.. Recommanded Product: 6-Chlorobenzothiazol-2-ylamine The article mentions the following:

Synthesis of a series of 2-arylbenzo[d]imidazo[2,1-b]thiazoles tethered with barbituric acid moiety was reported from the three component reaction of 2-aminobenzothiazoles, barbituric acids and terminal aryl acetylenes or aryl Me ketones in the presence of I2 in DMSO medium. Both conventional and microwave heating conditions was used for this multicomponent reaction. The salient features of this methodol. are: (i) formation of one C-C and two C-N bonds in one-pot under metal-free oxidation followed by cyclization (ii) selective formation of the fused imidazole ring, (iii) wide substrate scope (iv) easy purification of the products (v) products having more than one pharmaceutically important motifs and (vi) gram scale synthesis possible. The results came from multiple reactions, including the reaction of 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Recommanded Product: 6-Chlorobenzothiazol-2-ylamine)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Recommanded Product: 6-Chlorobenzothiazol-2-ylamineThiazoles can also be used as protected formyl groups, which can be released in later stages of complex natural product synthesis.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

《Metabolic characterization, antioxidant capacity, and In vitro antiurolithiatic potential of Achillea millefolium L.》 was published in Journal of Young Pharmacists in 2021. These research results belong to Kain, Dolly; Kumar, Suresh; Vandana; Suryavanshi, Amrita; Arya, Atul. Application In Synthesis of ABTS Diammonium The article mentions the following:

The current study investigated the antioxidant and antiurolithiatic potential of A. millefolium along with its metabolic characterization. A. millefolium has been collected at vegetative (V) and mature (Inflorescence (I) and Stem and Leaves (S+L)) stages and extracted in ethanol. Antioxidant potential has been determined by total phenolic content, total flavonoid content, ferric reducing antioxidant potential, total antioxidants through phosphomolybdate assay and free radical scavenging assays (1,1-diphenyl-2-picrylhydrazyl) and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid). In vitro antiurolithiatic activity is determined by turbidity changes in artificial urine, nucleation assay, and aggregation assay. Fourier transform-IR spectroscopy and gas chromatog.-mass spectrometry has been used to characterize the active metabolites of plant A. millefolium. Maximum antioxidant activity was observed in Inflorescence with values 76.58 mg of GAE/g of extract, 18.82 mg of RE/g of extract, 199.799 mg of AAE/g of extract and 327.95 mg of AAE/g of extract for TPC, TFC, FRAP and total antioxidants through phosphomolybdate assay resp. Maximum radical scavenging activity was also observed in inflorescence with 86.3% and 69.655 % inhibition against DPPH and ABTS free radicals at 1000μg/mL concentration resp. Maximum in vitro antiurolithiatic potential of plant A. millefolium observed in inflorescence i.e. 80 %, 41.84% and 63.41 % inhibition at 1000μg/mL concentration for turbidity changes in artificial urine method, nucleation assay and aggregation assay resp. FT- IR and GCMS of inflorescence of A. millefolium confirmed the presence of major functional groups and active metabolic compounds resp. Inflorescence of plant A. millefolium is an excellent source of natural antioxidants with significant antiurolithiatic potential. In the experiment, the researchers used many compounds, for example, ABTS Diammonium(cas: 30931-67-0Application In Synthesis of ABTS Diammonium)

ABTS Diammonium(cas: 30931-67-0) belongs to anime. To avoid the problem of multiple alkylation, methods have been devised for “blocking” substitution so that only one alkyl group is introduced. The Gabriel synthesis is one such method; it utilizes phthalimide, C6H4(CO)2NH, whose one acidic hydrogen atom has been removed upon the addition of a base such as KOH to form a salt.Application In Synthesis of ABTS Diammonium

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Electric Literature of C18H24N6O6S4On March 22, 2020, Thepchuay, Yanisa; Sonsa-ard, Thitaporn; Ratanawimarnwong, Nuanlaor; Auparakkitanon, Saranya; Sitanurak, Jirayu; Nacapricha, Duangjai published an article in Analytica Chimica Acta. The article was 《Paper-based colorimetric biosensor of blood alcohol with in-situ headspace separation of ethanol from whole blood》. The article mentions the following:

This work presents a novel development that exploits the concept of in-situ gas-separation together with a specific enzymic colorimetric detection to produce a portable biosensor called “”Blood Alc. Micro-pad”” for direct quantitation of ethanol in whole blood. The thin square device (25 mm × 25 mm × 1.8 mm) comprises two layers of patterned filter paper held together with a double-sided mounting tape with an 8-mm circular hole (the headspace). In operation, the reagent is deposited on one layer and covered with sticky tape. Then 8μL of a blood sample is dispensed onto the opposite layer and covered with sticky tape. Diffusion of ethanol across the 1.6 mm narrow headspace permits selective detection of ethanol by the enzymic reagents deposited on the opposite layer. This reagent zone contains alc. oxidase, horseradish peroxidase and 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, as the chromogenic reagent. The color intensity, measured from the recorded digital image, resulting from the enzymic assay of ethanol, correlates with the concentration of blood alc. The results obtained with spiked mice and sheep blood samples, using an external calibration in the range of 1-120 mg dL-1ethanol, gave recoveries of 93.2-104.4% (n = 12). The “”Blood Alc. Micro-pad”” gave good precision with %RSD <1 (50 mg dL-1 ethanol, n = 10) and limit of quantification (10SD of intercept/slope) of 11.56 mg dL-1. The method was successfully validated against a headspace gas chromatog.-mass spectrometric method. It has good potential for development as a simple and convenient blood alc. sensor for on-site testing. The results came from multiple reactions, including the reaction of ABTS Diammonium(cas: 30931-67-0Electric Literature of C18H24N6O6S4)

ABTS Diammonium(cas: 30931-67-0) belongs to anime. Large quantities of aliphatic amines are made synthetically. The most widely used industrial method is the reaction of alcohols with ammonia at a high temperature, catalyzed by metals or metal oxide catalysts (e.g., nickel or copper). Mixtures of primary, secondary, and tertiary amines are thereby produced.Electric Literature of C18H24N6O6S4

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Quality Control of 6-Chlorobenzothiazol-2-ylamineOn October 31, 2020 ,《Design and Synthesis of 2-Substituted Benzothiazole Derivatives as Antioxidant and Antimicrobial Agents》 was published in Current Bioactive Compounds. The article was written by Nishad, Ravi K.; Shukla, Karuna S.; Chawla, Pooja A.. The article contains the following contents:

An upsurge in the number of antibiotic-resistant microbial infections has warranted the discovery and development of new antibiotics. This is a matter of great concern for effective therapy for a search of novel antimicrobial agents. Literature has a number of reports of involvement of oxidative stress due to an imbalance between the generation and neutralization of free radicals in many diseases. Heterocyclic compounds have been involved in the treatment of various disorders. Benzothiazole is one such heterocyclic nucleus having benzene ring merged with the thiazole ring. Among the various substitutions possible in this nucleus, substitutions at position-2 have already been reported with potential bioactivities. Thus, different substituted compounds have been synthesized which could serve as antimicrobials and antioxidants. Benzothiazole derivatives (B1-B7) were synthesized by two-step reactions and the structures were confirmed through IR, mass and NMR spectroscopy. The compounds were evaluated for in vitro antioxidant and antimicrobial activities using standard methods. The results of antibacterial and antifungal activity showed that compound B4 exhibited maximum activity against all the tested strains of microorganisms with the zone of inhibition 17.1-18.5 mm and MIC value 1.1-1.5μg/mL. Compound B5 exhibited potent antioxidant activity. The compounds substituted with halogen on the aryl ring showed increased antimicrobial activity as seen in the case of compound B4 (6-fluoro). The compounds substituted with a hydroxyl group (B5) exhibited good antioxidant activity. The experimental part of the paper was very detailed, including the reaction process of 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Quality Control of 6-Chlorobenzothiazol-2-ylamine)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Quality Control of 6-Chlorobenzothiazol-2-ylamineThe thiazole ring has been identified as a central feature of numerous natural products, perhaps the most famous example of which is epothilone.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Bhoi, Manoj N.; Borad, Mayuri A.; Pithawala, Edwin A.; Patel, Hitesh D. published their research in Arabian Journal of Chemistry on December 31 ,2019. The article was titled 《Novel benzothiazole containing 4H-pyrimido[2,1-b]benzothiazoles derivatives: One-pot, solvent-free microwave assisted synthesis and their biological evaluation》.SDS of cas: 95-24-9 The article contains the following contents:

A series of pyrimido[2,1-b]benzothiazoles I [R = H, 6-Me, 8-Br, etc.] was synthesized via microwave-assisted one-pot three-component reaction of 2-aminobenzothiazoles with pyridine-2-aldehyde and Et acetoacetate in the presence of PdCl2 as an expeditious catalyst under solvent-free conditions. The salient features of this approach were operational simplicity, convergence, short reaction time, high atom economy, easy workup, mild reaction conditions and environmentally benign conditions. All the newly synthesized pyrimido[2,1-b]benzothiazole derivatives were evaluated for their antibacterial, antitubercular and antioxidant activities. Among the tested compounds, pyrimido[2,1-b]benzothiazoles I [R = 6-Me, 8-Me, 6-Cl, 8-Cl, 8-NO2] were found to be more active against tested Gram +ve and Gram -ve bacterial strains. Pyrimido[2,1-b]benzothiazoles I [R = 8-Me, 8-NO2] showed very good % inhibition 72.9% and 70.32% resp. against Mycobacterium tuberculosis H37RV whereas remaining compounds displayed moderate to good antitubercular activity. All the pyrimido[2,1-b]benzothiazoles I showed moderate to good antioxidant activity. The experimental part of the paper was very detailed, including the reaction process of 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9SDS of cas: 95-24-9)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.SDS of cas: 95-24-9The thiazole ring has been identified as a central feature of numerous natural products, perhaps the most famous example of which is epothilone.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Chen, Xue-Ping; Hou, Ke-Qiang; Zhou, Feng; Chan, Albert S. C.; Xiong, Xiao-Feng published an article on January 15 ,2021. The article was titled 《Organocatalytic Asymmetric Synthesis of Benzothiazolopyrimidines via [4 + 2] Cyclization of 2-Benzothiazolimines and Aldehydes》, and you may find the article in Journal of Organic Chemistry.Application In Synthesis of 6-Chlorobenzothiazol-2-ylamine The information in the text is summarized as follows:

The direct asym. synthesis of pyrimido[2,1-b]benzothiazoles using a com. available chiral amine catalyst was reported. A variety of 2-benzothiazolimines and aldehydes were well tolerated under the reaction conditions and generated the corresponding products in 81-99% yields with excellent diastereoselectivities and enantioselectivities (up to >20:1 dr, 99% ee). Furthermore, the products could be easily converted to other useful chiral building blocks. The experimental part of the paper was very detailed, including the reaction process of 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Application In Synthesis of 6-Chlorobenzothiazol-2-ylamine)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Application In Synthesis of 6-Chlorobenzothiazol-2-ylamineThe thiazole ring has been identified as a central feature of numerous natural products, perhaps the most famous example of which is epothilone.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

In 2013,New Journal of Chemistry included an article by Zhou, Xiaojing; Zhang, Zhijuan; Li, Baiyan; Yang, Fen; Peng, Yu; Li, Guanghua; Shi, Zhan; Feng, Shouhua; Li, Jing. Electric Literature of C10H8N2O2S. The article was titled 《Two three-dimensional metal-organic frameworks constructed by thiazole-spaced pyridinecarboxylates exhibiting selective gas sorption or antiferromagnetic coupling》. The information in the text is summarized as follows:

The ligand of 2-(4-pyridyl)-4-methylthiazole-5-carboxylic acid (HL) was employed to react with Cd(NO3)2·6H2O or a 50% aqueous solution of Mn(NO3)2, to yield two isomorphous three-dimensional (3D) coordination compounds, [Cd(L)2](H2O)4(DMF) (1) and [Mn(L)2](H2O)3(DMF) (2). The structures and properties of the compounds were characterized by several techniques. Both compounds are three-dimensional (3D) neutral frameworks possessing permanent pores that give rise to straight 1-dimensional channels. Compound 1 displays adsorption selectivity for CO2, while the low pressure sorption of H2, N2, CH4, CO, O2 at 298 K are negligible; Compound 2 shows weak anti-ferromagnetic coupling between the magnetic centers. After reading the article, we found that the author used 4-Methyl-2-(pyridin-4-yl)thiazole-5-carboxylic acid(cas: 144060-98-0Electric Literature of C10H8N2O2S)

4-Methyl-2-(pyridin-4-yl)thiazole-5-carboxylic acid(cas: 144060-98-0) belongs to pyridine. Pyridine, its benzo and pyridine-based compounds play diverse roles in organic chemistry. As ligands, solvents, and catalysts they facilitate reactions; thus descriptions of these new ligands and their applications abound each year.Electric Literature of C10H8N2O2S

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Majerska, Monika; Jakubec, Martin; Klimsa, Vojtech; Rimpelova, Silvie; Kral, Vlastimil; Stepanek, Frantisek published an article in Molecular Pharmaceutics. The title of the article was 《Microgel bioreactors for cancer cell targeting by pH-dependent generation of radicals》.Quality Control of ABTS Diammonium The author mentioned the following in the article:

The lack of specificity of traditional cytostatics and increasing resistance of cancer cells represent important challenges in cancer therapy. One of the characteristics of cancer cells is their intrinsic oxidative stress caused by higher metabolic activity, mitochondrial malfunction, and oncogene stimulation. This feature can be exploited in the pursuit of more selective cancer therapy, as there is increasing evidence that cancer cells are more sensitive to elevated concentrations of reactive oxygen species than normal cells. In this study, we demonstrate a new concept for cancer cell targeting by in situ production of radicals under physiol. conditions. The biol. active radicals are produced in the milieu of cancer cells by enzymic conversion from an inactive precursor, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt, by using miniature bioreactors represented by cell-sized microgels containing immobilized laccase. We utilize the pH-dependent activity of laccase to generate radicals only at a lower pH (5.7-6.1) that is characteristic of the tumor microenvironment. The composition of the microgels was optimized so as to allow sufficient substrate and radical diffusion, high enzyme activity, and stability under physiol. conditions. The functionality of this system was evaluated on three cancer cell lines (HeLa, HT-29, and DLD1) and the cytotoxicity of in situ-produced radicals was successfully proven in all cases. These results demonstrate that cancer cell targeting by in situ-generated radicals using miniature enzymic reactors may represent an alternative to traditional cytostatics. In particular, the pH-dependence of radical generation and their short-lived nature can ensure localized functionality in the tumor microenvironment and thereby reduce systemic side-effects. In the part of experimental materials, we found many familiar compounds, such as ABTS Diammonium(cas: 30931-67-0Quality Control of ABTS Diammonium)

ABTS Diammonium(cas: 30931-67-0) belongs to anime. Left-handed and right-handed forms (mirror-image configurations, known as optical isomers or enantiomers) are possible when all the substituents on the central nitrogen atom are different (i.e., the nitrogen is chiral). With amines, there is extremely rapid inversion in which the two configurations are interconverted.Quality Control of ABTS Diammonium

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

The author of 《Spindle and kinetochore-associated complex subunit 3 (SKA3) promotes stem cell-like properties of hepatocellular carcinoma cells through activating Notch signaling pathway》 were Bai, Shuya; Chen, Wei; Zheng, Mengli; Wang, Xiju; Peng, Wang; Zhao, Yuchong; Wang, Yun; Xiong, Si; Cheng, Bin. And the article was published in Annals of Translational Medicine in 2021. Synthetic Route of C7H5ClN2S The author mentioned the following in the article:

Cancer stemness contributes to hepatocellular carcinoma (HCC) initiation, metastasis, drug resistance, and recurrence. The spindle and kinetochore-associated (SKA) complex has been shown to be involved in tumor progression; however, its effects on cancer stem cell-like properties have not yet been examined This research sought to study each subunit of the SKA complex in HCC systematically. Bioinformatic analyses were carried out to examine the expression and clin. data of the SKA complex′s each subunit in HCC. The expression of the target genes was detected by quant. reverse transcription-polymerase chain reaction and Western blot assays. Clone formation and Transwell assays were performed to assess the proliferation and migration abilities of the SKA complex′s each subunit. Sphere formation assays and s.c. xenograft experiments were performed to investigate the effects of SKA complex subunit 3 (SKA3) on the self-renewal and tumorigenic abilities of HCC. Each subunit of the SKA complex was highly expressed in HCC, but only SKA complex subunit 1 (SKA1) and SKA3 were associated with the poor overall survival of HCC patients. Addnl., the HCC cells overexpressing SKA3 exhibited increased migration, invasion, proliferation, self-renewal, Sorafenib resistance and tumorigenic abilities. Notch signaling played a vital role in the process by which SKA3 promoted HCC stemness. SKA3 promotes HCC stem cell-like properties via the Notch signaling pathway. As SKA3 appears to act as a regulator of stemness in HCC, it might be a potential mol. target for HCC. The experimental process involved the reaction of 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Synthetic Route of C7H5ClN2S)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Synthetic Route of C7H5ClN2STheir presence in peptides or their ability to bind proteins, DNA and RNA has led to many synthetic studies and new applications.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica