Day: October 20, 2022

Quality Control of ABTS DiammoniumOn May 31, 2021, Yan, Bingsong; Liu, Wendong; Duan, Guangbin; Ni, Pengjuan; Jiang, Yuanyuan; Zhang, Chenghui; Wang, Bo; Lu, Yizhong; Chen, Chuanxia published an article in Microchimica Acta. The article was 《Colorimetric detection of acetylcholinesterase and its inhibitor based on thiol-regulated oxidase-like activity of 2D palladium square nanoplates on reduced graphene oxide》. The article mentions the following:

A convenient and sensitive colorimetric assay for acetylcholinesterase (AChE) and its inhibitor has been designed based on the oxidase-like activity of {100}-faceted Pd square nanoplates which are grown in situ on reduced graphene oxide (PdSP@rGO). PdSP@rGO can effectively catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) without the assistance of H2O2 to generate blue oxidized TMB (oxTMB) with a characteristic absorption peak at 652 nm. In the presence of AChE, acetylthiocholine (ATCh), a typical AChE substrate, is hydrolyzed to thiocholine (TCh). The generated TCh can effectively inhibit the PdSP@rGO-triggered chromogenic reaction of TMB via cheating with Pd, resulting in color fading and decrease in absorbance. Thus, a sensitive probe for AChE activity is constructed with a working range of 0.25-5 mU mL-1 and a limit of detection (LOD) of 0.0625 mU mL-1. Furthermore, because of the inhibition effect of tacrine on AChE, tacrine is also detected through the colorimetric AChE assay system within the concentrations range 0.025-0.4μM with a LOD of 0.00229μM. Hence, a rapid and facile colorimetric procedure to sensitively detect AChE and its inhibitor can be anticipated through modulating the oxidase-like activity of [email protected] Diammonium(cas: 30931-67-0Quality Control of ABTS Diammonium) was used in this study.

ABTS Diammonium(cas: 30931-67-0) belongs to anime. Reduction of nitro compounds, RNO2, by hydrogen or other reducing agents produces primary amines cleanly (i.e., without a mixture of products), but the method is mostly used for aromatic amines because of the limited availability of aliphatic nitro compounds. Reduction of nitriles and oximes (R2C=NOH) also yields primary amines.Quality Control of ABTS Diammonium

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Category: thiazoleOn March 26, 2020, Singh, Manjula; Dhar S. Yadav, Lal; Krishna Pal Singh, Rana published an article in Tetrahedron Letters. The article was 《Visible-light photoredox catalytic approach for the direct synthesis of 2-aminobenzothiazoles from anilines》. The article mentions the following:

A novel, highly efficient and convenient approach for the visible-light-promoted direct synthesis of 2-aminobenzothiazoles from anilines and ammonium thiocyanate is presented. The reaction involves addition/cyclization cascade of SCN radical and anilines under photoredox catalysis with Ru(bpy)3Cl2. The salient features of the protocol include the utilization of atm. oxygen and visible light as clean, inexpensive and sustainable resources at room temperature After reading the article, we found that the author used 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Category: thiazole)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Category: thiazoleThiazoles can also be used as protected formyl groups, which can be released in later stages of complex natural product synthesis.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Name: 6-Chlorobenzothiazol-2-ylamineOn October 31, 2019 ,《A novel assay to screen siRNA libraries identifies protein kinases required for chromosome transmission》 appeared in Genome Research. The author of the article were Liskovykh, Mikhail; Goncharov, Nikolay V.; Petrov, Nikolai; Aksenova, Vasilisa; Pegoraro, Gianluca; Ozbun, Laurent L.; Reinhold, William C.; Varma, Sudhir; Dasso, Mary; Kumeiko, Vadim; Masumoto, Hiroshi; Earnshaw, William C.; Larionov, Vladimir; Kouprina, Natalay. The article conveys some information:

One of the hallmarks of cancer is chromosome instability (CIN), which leads to aneuploidy, translocations, and other chromosome aberrations. However, in the vast majority of human tumors the mol. basis of CIN remains unknown, partly because not all genes controlling chromosome transmission have yet been identified. To address this question, we developed an exptl. high-throughput imaging (HTI) siRNA assay that allows the identification of novel CIN genes. Our method uses a human artificial chromosome (HAC) expressing the GFP transgene. When this assay was applied to screen an siRNA library of protein kinases, we identified PINK1, TRIO, IRAK1, PNCK, and TAOK1 as potential novel genes whose knockdown induces various mitotic abnormalities and results in chromosome loss. The HAC-based assay can be applied for screening different siRNA libraries (cell cycle regulation, DNA damage response, epigenetics, and transcription factors) to identify addnl. genes involved in CIN. Identification of the complete spectrum of CIN genes will reveal new insights into mechanisms of chromosome segregation and may expedite the development of novel therapeutic strategies to target the CIN phenotype in cancer cells. After reading the article, we found that the author used 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Name: 6-Chlorobenzothiazol-2-ylamine)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Name: 6-Chlorobenzothiazol-2-ylamineThiazoles can also be used as protected formyl groups, which can be released in later stages of complex natural product synthesis.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Tokala, Ramya; Mahajan, Surbhi; Kiranmai, Gaddam; Sigalapalli, Dilep Kumar; Sana, Sravani; John, Stephy Elza; Nagesh, Narayana; Shankaraiah, Nagula published an article on January 31 ,2021. The article was titled 《Development of β-carboline-benzothiazole hybrids via carboxamide formation as cytotoxic agents: DNA intercalative topoisomerase IIα inhibition and apoptosis induction》, and you may find the article in Bioorganic Chemistry.Computed Properties of C7H5ClN2S The information in the text is summarized as follows:

In quest of promising anticancer agents, the pharmacophores of natural (β-carboline) and synthetic origin (benzothiazole) were adjoined by a carboxamide bridge and three-point diversification was accomplished. The in vitro cytotoxic ability of the compounds was established on adherent and suspension human cancer cell lines and compounds I and II advanced as pre-eminent mols. with IC50 values of 1.46 and 1.81μM resp. in A549 cell line. The cytospecificity was entrenched for potent compounds I and II by evaluating against normal human lung epithelial cells and selectivity index was calculated Furthermore, EtBr displacement, relative viscosity and gel-based topoisomerase II target assays unveiled the intercalative topo-II inhibitory capability and DNA binding studies (absorbance) revealed the dissociation constant (Kd) for compounds I and II as 98 and 103μM resp. Addnl., cell-based flow cytometric assays like Annexin-V/PI dual staining aids in the quantification of apoptosis induced and JC-1 staining disclosed the depolarization of mitochondrial membrane potential by compound I in A549 cells in a dose-dependent manner. Moreover, wound healing assay established the inhibition of in vitro cell migration by compound I on A549 cells. In addition, mol. docking studies proved the binding of compounds I and II in the active site of DNA complexed with topo IIα and stabilized by interactions with DNA base pairs and amino acid residues. Remarkably, the compounds I and II follow Lipinski’s rule of five and are in the recommended range for Jorgensen’s rule of three with a minimal violation and other pharmacokinetic parameters revealing druggability of the synthesized hybrids. In the experiment, the researchers used 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Computed Properties of C7H5ClN2S)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Computed Properties of C7H5ClN2STheir presence in peptides or their ability to bind proteins, DNA and RNA has led to many synthetic studies and new applications.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

《A facile colorimetric sensor for ultrasensitive and selective detection of Lead(II) in environmental and biological samples based on intrinsic peroxidase-mimic activity of WS2 nanosheets》 was written by Tang, Yue; Hu, Yang; Yang, Yuxia; Liu, Bangyan; Wu, Yuangen. Synthetic Route of C18H24N6O6S4 And the article was included in Analytica Chimica Acta on April 15 ,2020. The article conveys some information:

Two-dimensional layered WS2 nanosheets with rich active edge exhibit intrinsic peroxidase-mimic activity, which make them an ideal material for sensor design. However, there is still lack of research on the catalysis and regulation mechanisms of the layered WS2 nanosheets as well as their application in the detection of hazardous substances. Herein, the regulatory effect of Pb(II) on the peroxidase-mimic activity of the layered WS2 nanosheets was firstly investigated, which enable us to construct a novel and facile colorimetric sensor for ultrasensitive and selective detection of Pb(II). To improve the performance of colorimetric sensor, some important parameters like buffer conditions, substrates and temperature have been investigated. Under the optimal conditions, the catalytic kinetics of layered WS2 nanosheets were extensively investigated. The peroxidase-mimic catalytic reaction was proved to be the “”ping pong”” mechanism, and the regulatory effect of Pb(II) on layered WS2 nanosheets was agreed with noncompetitive inhibition. The absorbance variation of colorimetric sensor is proportionally related to the concentration of heavy metals, which enable us to easily distinguish whether Pb(II) exceeds the permissible level in less than 20 min even by the naked eyes. The limit of detection (LOD) and the limit of quantification (LOQ) of the proposed colorimetric sensor for Pb(II) were determined as low as 4μg L-1 and 13.3μg L-1, and displays excellent selectivity against other competitive metal ions. Moreover, the further studies also validate the applicability of colorimetric sensor in several actual samples, indicating that our strategy may has prospective applications for Pb(II) detection in environment and biol. samples.ABTS Diammonium(cas: 30931-67-0Synthetic Route of C18H24N6O6S4) was used in this study.

ABTS Diammonium(cas: 30931-67-0) belongs to anime.Typically the presence of an amine functional group is deduced by a combination of techniques, including mass spectrometry as well as NMR and IR spectroscopies. 1H NMR signals for amines disappear upon treatment of the sample with D2O. In their infrared spectrum primary amines exhibit two N-H bands, whereas secondary amines exhibit only one.Synthetic Route of C18H24N6O6S4

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Iwata, Satoru; Aoyama, Misa; Uchida, Satoshi; Tanaka, Kiyoshi published their research in Chemistry Letters on December 5 ,2012. The article was titled 《Novel 4-trifluoromethylthiazole-5-carboxylic acid as acceptor in photosensitized dyes》.Reference of 4-(Trifluoromethyl)thiazole-5-carboxylic acid The article contains the following contents:

Novel photosensitized dyes containing 4-trifluoromethylthiazole-5-carboxylic acid as an acceptor are synthesized. Stilbene-type dyes with this acceptor show high performance as dye-sensitized solar cells. The trifluoromethyl group is assumed to act as a suppressor of electron back-donation from the TiO2 conduction band to the electrolyte and as an accelerator of charge separation in the photoexcited state. After reading the article, we found that the author used 4-(Trifluoromethyl)thiazole-5-carboxylic acid(cas: 167548-89-2Reference of 4-(Trifluoromethyl)thiazole-5-carboxylic acid)

4-(Trifluoromethyl)thiazole-5-carboxylic acid(cas: 167548-89-2) is a member of organofluorine compounds. Organofluorine compounds, which have carbon-fluorine bonds, show unique features such as high thermal and chemical stability, high surface activity, no light-absorbing ability, high pharmacological effect, and so on. Owing to their specific characters, they are indispensable chemicals for industry and our daily lives.Reference of 4-(Trifluoromethyl)thiazole-5-carboxylic acid

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Zhang, Yan; Li, Yuxin; Li, Huan; Shang, Junfeng; Li, Zhengming; Wang, Baolei published an article on January 31 ,2022. The article was titled 《Synthesis and insecticidal evaluation of novel sulfide-containing amide derivatives as potential ryanodine receptor modulators》, and you may find the article in Chinese Chemical Letters.Quality Control of 6-Chlorobenzothiazol-2-ylamine The information in the text is summarized as follows:

With the aim of discovering new bioactive pesticides for crop protection, a series of novel sulfide-containing amide derivatives A were efficiently synthesized via a strategy of modifying the “”amide”” structure of anthranilic diamide insecticides. The single-crystal structures of A2-3 and A4-5 were firstly reported. The bioassay results showed that most of the synthesized compounds display moderate to high insecticidal activities. Particularly, some sulfone-containing compounds, e.g.,A2-3,A3-3 and A6-3, not only possessed favorable lethality rate (50%-100%) against P. xylostella at a concentration of 0.1 mg/L, but also held good activities towards a variety of agricultural pests such as M. separata, C. pipiens pallen, H. armigera and O. nubilalis; the larvicidal activities of A4-1 and A6-1 towards P. xylostella were close to that of chlorantraniliprole at 0.01 mg/L. The calcium imaging experiments revealed that the representative compounds A2-3 and A6-3 are potential ryanodine receptor (RyR) modulators. The structure-activity relationships were discussed in detail. These results provide useful information for further design and development of novel insecticides. In the experiment, the researchers used many compounds, for example, 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Quality Control of 6-Chlorobenzothiazol-2-ylamine)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Quality Control of 6-Chlorobenzothiazol-2-ylamineTheir presence in peptides or their ability to bind proteins, DNA and RNA has led to many synthetic studies and new applications.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

《Identification of N-benzothiazolyl-2-benzenesulfonamides as novel ABCA1 expression upregulators》 was published in RSC Medicinal Chemistry in 2020. These research results belong to Liu, Hongtao; Jiang, Xinhai; Gao, Xinfeng; Tian, Wenhua; Xu, Chen; Wang, Ruizhi; Xu, Yanni; Wei, Liping; Cao, Feng; Li, Wenyan. Reference of 6-Chlorobenzothiazol-2-ylamine The article mentions the following:

ATP binding cassette transporter A1 (ABCA1) is a critical transporter that mediates cellular cholesterol efflux from macrophages to apolipoprotein A-I (ApoA-I). Therefore, increasing the expression level of ABCA1 is anti-atherogenic and ABCA1 expression upregulators have become novel choices for atherosclerosis treatment. In this study, a series of N-benzothiazolyl-2-benzenesulfonamides, based on the structure of WY06 discovered in our laboratory, were designed and synthesized as novel ABCA1 expression upregulators. Based on an in vitro ABCA1 upregulatory cell model, ABCA1 upregulation of target compounds was evaluated. Compounds 6c, 6d, and 6i have good upregulated ABCA1 expression activities, with EC50 values of 0.97, 0.37, and 0.41μM, resp. A preliminary structure-activity relationship is summarized. Replacing the methoxy group on the benzothiazole moiety of WY06 with a fluorine or chlorine atom and exchanging the ester group with a cyano group resulted in more potent ABCA1 upregulating activity. Moreover, compound 6i increased ABCA1 mRNA and protein expression and significantly promoted cholesterol efflux in RAW264.7 cells. In conclusion, N-benzothiazolyl-2-benzenesulfonamides were identified as novel ABCA1 expression upregulators. In the experimental materials used by the author, we found 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Reference of 6-Chlorobenzothiazol-2-ylamine)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Reference of 6-Chlorobenzothiazol-2-ylamineThiazoles can also be used as protected formyl groups, which can be released in later stages of complex natural product synthesis.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

《Regioselective synthesis of benzo[b]indeno[1,2-e][1,4]thiazin-11(10aH)-ones and antimicrobial evaluation thereof》 was published in Synthetic Communications in 2022. These research results belong to Mor, Satbir; Khatri, Mohini. Electric Literature of C7H5ClN2S The article mentions the following:

Benzo[b]indeno[1,2-e][1,4]thiazin-11(10aH)-ones I [R = 2-F, 3-F, 4-Cl; R1 = H, Cl, Br, Me, MeO] were synthesized regioselectively via cyclocondensation of 2,5-dibromo-2-(substitutedphenyl)-1H-indene-1,3(2H)-diones and 2-aminobenzenethiols using freshly dried EtOH as solvent with 42-60% yields. The synthesized derivatives I were well-characterized by employing different spectral techniques (FTIR, 1H and 13C NMR, and HRMS) and X-ray crystallog. anal. All the derivatives I were examined for their preliminary in vitro antimicrobial activity against two Gram-pos. bacterial strains, i.e., B. subtilis, and S. aureus, two Gram-neg. bacterial strains, i.e., P. aeruginosa and E. coli, and two fungal strains, i.e., C. albicans and R. oryzae employing serial dilution method taking Ciprofloxacin and Fluconazole as standard reference drugs for antibacterial and antifungal activities, resp. Among all the tested derivatives I, derivative I [R = 4-Cl; R1 = Br] was found to exhibit significant inhibition against all the microbial strains under study. In the experimental materials used by the author, we found 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Electric Literature of C7H5ClN2S)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Electric Literature of C7H5ClN2SThiazoles can also be used as protected formyl groups, which can be released in later stages of complex natural product synthesis.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Formula: C7H5ClN2SOn May 31, 2022, P, Keerthana; Abbo, Hanna; Cherian, Anila Rose; Titinchi, Salam; Varghese, Anitha published an article in Journal of Fluorescence. The article was 《An Efficient Inclusion Complex Based Fluorescent Sensor for Mercury (II) and its Application in Live-Cell Imaging》. The article mentions the following:

The formation of an inclusion complex between hydroxypropyl-β-cyclodextrin (H-CD) and 4-acetylphenyl-4-(((6-chlorobenzo[d]thiazol-2-yl)-imino)-methyl)-benzoate (L) was investigated by FT-IR, 1H-NMR, X-ray diffraction (XRD), FT-Raman, scanning electron microscope (SEM) techniques in the solid-state, absorption and emission spectroscopy in the liquid state and the virtual state as mol. docking technique. The binding properties of the inclusion complex (H-CD: L) with cations in deionized water was observed via absorbance and photoluminescence (PL) emission spectroscopy. The fluorescence probe (H-CD: L) inclusion complex (IC) was examined for several heavy metal cations, and identified that the PL emission wavelength of the complex displayed a continuous rise in the fluorescence intensity for Hg2+. A linearity range of 1 x 10-8 – 11 x 10-8 M and limit of detection value of 2.71 x 10-10 M was found to be achieved for the detection of Hg2+. This outcome proves that the inclusion complex H-CD: L would be a promising material for the development a solid-state fluorescence probe for detecting Hg2+. It also shows application in real sample anal. and cell imaging. In addition to this study using 6-Chlorobenzothiazol-2-ylamine, there are many other studies that have used 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Formula: C7H5ClN2S) was used in this study.

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Formula: C7H5ClN2SThiazoles can also be used as protected formyl groups, which can be released in later stages of complex natural product synthesis.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica