Spunde, Karina; Vigante, Brigita; Dubova, Unda Nelda; Sipola, Anda; Timofejeva, Irena; Zajakina, Anna; Jansons, Juris; Plotniece, Aiva; Pajuste, Karlis; Sobolev, Arkadij; Muhamadejev, Ruslan; Jaudzems, Kristaps; Duburs, Gunars; Kozlovska, Tatjana published an article in 2022, the title of the article was Design and Synthesis of Hepatitis B Virus (HBV) Capsid Assembly Modulators and Evaluation of Their Activity in Mammalian Cell Model.COA of Formula: C5H5NOS And the article contains the following content:
Capsid assembly modulators (CAMs) have emerged as a promising class of antiviral agents. Herein, the effects of twenty-one newly designed and synthesized CAMs including (heteroaryl)dihydropyrimidines (HAPs), their analogs and standard compounds on hepatitis B virus (HBV) capsid assembly have been studied. Cytoplasmic expression of the HBV core (HBc) gene driven by the exogenously delivered recombinant alphavirus RNA replicon was used for high level production of the full-length HBc protein in mammalian cells. HBV capsid assembly was assessed by native agarose gel immunoblot anal., electron microscopy and inhibition of virion secretion in HepG2.2.15 HBV producing cell line. Induced fit docking simulation was applied for modeling the structural relationships of the synthesized compounds and HBc. The most efficient were the HAP class compounds, dihydropyrimidine-5-carboxylic acid n-alkoxyalkyl esters, which induced the formation of incorrectly assembled capsid products and their accumulation within the cells. HBc product accumulation in the cells was not detected with the reference HAP compound Bay 41-4109, suggesting different modes of action. A significant antiviral effect and substantially reduced toxicity were revealed for two of the synthesized compounds Two new HAP compounds revealed a significant antiviral effect and a favorable toxicity profile that allows these compounds to be considered promising leads and drug candidates for the treatment of HBV infection. The experimental process involved the reaction of 2-Acetylthiazole(cas: 24295-03-2).COA of Formula: C5H5NOS
The Article related to dihydropyrimidine heteroaryl preparation hepatitis virus capsid assembly modulator antitumor, bay 41-4109, antivirals, capsid assembly, capsid assembly modulator, full-length hbv core, hepatitis b virus, heteroaryldihydropyrimidines and other aspects.COA of Formula: C5H5NOS
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica