Month: October 2022

《Discovery of MFH290: A Potent and Highly Selective Covalent Inhibitor for Cyclin-Dependent Kinase 12/13》 was published in Journal of Medicinal Chemistry in 2020. These research results belong to Liu, Yao; Hao, Mingfeng; Leggett, Alan L.; Gao, Yang; Ficarro, Scott B.; Che, Jianwei; He, Zhixiang; Olson, Calla M.; Marto, Jarrod A.; Kwiatkowski, Nicholas P.; Zhang, Tinghu; Gray, Nathanael S.. Quality Control of 5-Bromothiazol-2-amine The article mentions the following:

Genetic depletion of cyclin-dependent kinase 12 (CDK12) or selective inhibition of an analog-sensitive CDK12 reduces DNA damage repair gene expression, but selective inhibition of endogenous CDK12 is difficult. Here, we report the development of MFH290(I), a novel cysteine (Cys)-directed covalent inhibitor of CDK12/13. MFH290 forms a covalent bond with Cys-1039 of CDK12, exhibits excellent kinome selectivity, inhibits the phosphorylation of serine-2 in the C-terminal domain (CTD) of RNA-polymerase II (Pol II), and reduces the expression of key DNA damage repair genes. Importantly, these effects were demonstrated to be CDK12-dependent as mutation of Cys-1039 rendered the kinase refractory to MFH290 and restored Pol II CTD phosphorylation and DNA damage repair gene expression. Consistent with its effect on DNA damage repair gene expression, MFH290 augments the antiproliferative effect of the PARP inhibitor olaparib.5-Bromothiazol-2-amine(cas: 3034-22-8Quality Control of 5-Bromothiazol-2-amine) was used in this study.

5-Bromothiazol-2-amine(cas: 3034-22-8) belongs to anime. Many important products require amines as part of their syntheses. Methylamine is utilized in the production of the analgesic meperidine (trade name Demerol) and the photographic developer Metol (trademark), and dimethylamine is used in the synthesis of the antihistamine diphenhydramine (trade name Benadryl), the solvent dimethylformamide (DMF), and the rocket propellant 1,1-dimethylhydrazine. The synthesis of the insect repellent N,N-diethyl-m-toluamide (DEET) incorporates diethylamine while that of the synthetic fibre Kevlar requires aromatic amines.Quality Control of 5-Bromothiazol-2-amine

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

In 2021,Pharmaceuticals included an article by Stanciauskaite, Monika; Marksa, Mindaugas; Babickaite, Lina; Majiene, Daiva; Ramanauskiene, Kristina. Quality Control of ABTS Diammonium. The article was titled 《Comparison of Ethanolic and Aqueous Populus balsamifera L. Bud Extracts by Different Extraction Methods: Chemical Composition, Antioxidant and Antibacterial Activities》. The information in the text is summarized as follows:

The balsam poplar (Populus balsamifera L.) buds that grow in Lithuania are a polyphenol-rich plant material with a chem. composition close to that of propolis. In order to potentially adapt the extracts of this plant’s raw material for therapeutic purposes, it is important to carry out detailed studies on the chem. composition and biol. activity of balsam poplar buds. An important step is to evaluate the yield of polyphenols by different extraction methods and using different solvents. According to our research, extracts of balsam poplar buds collected in Lithuania are dominated by p-coumaric (496.9-13,291.2μg/g), cinnamic acid (32.9-11,788.5μg/g), pinobanksin (34.9-1775.5μg/g) and salicin (215.3-1190.7μg/g). The antioxidant activity of poplar buds was evaluated by the ABTS (2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric-reducing antioxidant power) methods, all extracts showed antioxidant activity and the obtained results correlated with the obtained amounts of total phenolic compounds in the extracts (ABTS r = 0.974; DPPH r = 0.986; FRAP r = 0.955, p <0.01). Studies of antimicrobial activity have shown that ethanolic extracts have an antimicrobal activity effect against Staphylococcus aureus, Enterococcus faecalis and Escherichia coli. The extracts showed a better antimicrobal activity against gram-pos. bacteria. In the part of experimental materials, we found many familiar compounds, such as ABTS Diammonium(cas: 30931-67-0Quality Control of ABTS Diammonium)

ABTS Diammonium(cas: 30931-67-0) belongs to anime. Amines characteristically form salts with acids; a hydrogen ion, H+, adds to the nitrogen. With the strong mineral acids (e.g., H2SO4, HNO3, and HCl), the reaction is vigorous. Salt formation is instantly reversed by strong bases such as NaOH. Neutral electrophiles (compounds attracted to regions of negative charge) also react with amines; alkyl halides (R′X) and analogous alkylating agents are important examples of electrophilic reagents.Quality Control of ABTS Diammonium

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

The author of 《Catalyst-free oxidative N-N coupling for the synthesis of 1,2,3-triazole compounds with tBuONO》 were Cai, Yue-Ming; Zhang, Xin; An, Cui; Yang, Ye-Fei; Liu, Wei; Gao, Wen-Xia; Huang, Xiao-Bo; Zhou, Yun-Bing; Liu, Miao-Chang; Wu, Hua-Yue. And the article was published in Organic Chemistry Frontiers in 2019. Application of 95-24-9 The author mentioned the following in the article:

A catalyst-free method was developed to synthesize 1,2,3-benzotriazinones I [R = H, 6-Cl, 6-Br, etc.] and 1,2,3-benzothiadiazoles II [R1 = H, 6-Cl, 6-Br, etc.] from 2-amino-benzamides and 2-amino thiols. Of particular note was that the one-step synthesis route to access [1,2,3]triazolo[1,5-a]pyridines III [R2 = H, Ph, 4-ClC6H4; R3 = H, 7-Me, 7-Br] from pyridin-2-ylmethanamine was reported for the first time. This approach featured no use of catalyst, extremely mild conditions and excellent efficiency. In addition to this study using 6-Chlorobenzothiazol-2-ylamine, there are many other studies that have used 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Application of 95-24-9) was used in this study.

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Application of 95-24-9The thiazole ring has been identified as a central feature of numerous natural products, perhaps the most famous example of which is epothilone.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

The author of 《Comparison of the content of flavonoids, total phenols, and carotenoids and antioxidant activity in Guang Citri reticulatae pericarpium during the aging time》 were Wang, Guangning; Chen, Shuwen; Gu, Guangshan; Qiu, Jieqing; Chen, Yuanling; Jia, Zhen; Tang, Hongmei. And the article was published in Pharmacognosy Magazine in 2020. COA of Formula: C18H24N6O6S4 The author mentioned the following in the article:

GCRP must be aged by stored for several years before it can be used as medicine. In the aging process, content of composition, antioxidant activity, and color of GCRP have changed. The objective of this study was to compare the content of flavonoids, total phenols, and carotenoids and antioxidant activity in GCRP during the aging time and analyze the correlations of the data. 40 Batches of GCRP in different aging years were used for qual. and quant. anal. of flavonoids, total phenols, and carotenoids by ultra-high-performance liquid chromatog. (HPLC)-mass spectrometry, HPLC-diode array detector, and UV methods. 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt and 1,1-diphenyl-2-trinitrophenylhydrazine methods were used to compare the antioxidant activity of GCRP. Colorimetric anal. method was used to detect the external color of GCRP. SPSS software was used to analyze the correlation of all data. With the increase of aging year, the content of three flavonoids and total phenols increased and the content of carotenoids decreased. The results showed that the antioxidant activity of ether extract increased significantly with aging year and methanol extract decreased. The correlation results showed that the content of carotenoids was more closely related to chromaticity values and antioxidant activity. The result provides more objective and detailed information to analyze the quality of GCRP during the aging time. After reading the article, we found that the author used ABTS Diammonium(cas: 30931-67-0COA of Formula: C18H24N6O6S4)

ABTS Diammonium(cas: 30931-67-0) belongs to anime. Halogenation, in which one or more hydrogen atoms of an amine is replaced by a halogen atom, occurs with chlorine, bromine, and iodine, as well as with some other reagents, notably hypochlorous acid (HClO). With primary amines the reaction proceeds in two stages, producing N-chloro- and N,N-dichloro-amines, RNHCl and RNCl2, respectively. With tertiary amines, an alkyl group may be displaced by a halogen.COA of Formula: C18H24N6O6S4

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

The author of 《Eelectrosynthesis of benzothiazole derivatives via C-H thiolation》 were Ahdenov, Reza; Mohammadi, Ali Asghar; Makarem, Somayeh; Taheri, Salman; Mollabagher, Hoda. And the article was published in Heterocyclic Communications in 2022. Safety of 6-Chlorobenzothiazol-2-ylamine The author mentioned the following in the article:

Benzothiazole derivatives are essential intermediates in synthesizing a wide variety of medical and pharmaceutical compounds, and there is a great demand for a simple and efficient method to synthesize benzothiazoles under mild reaction conditions. Organic electrosynthesis as an energy-efficient process represents an environmentally benign and safer method than traditional methods for organic synthesis. Herein, authors present bromine-free and straightforward synthesis of 2-amino benzothiazole derivatives via the reaction of aniline derivatives and ammonium thiocyanate using electrosynthesis in the presence of sodium bromide both as an electrolyte and as a brominating agent at room temperature in iso-Pr alc. (i-PrOH) as a solvent. The reaction of ammonium thiocyanate via C-H thiolation routes, using various aniline derivatives, resulted in a simple, green, and bromine-free synthesis of 2-amino benzothiazole in moderate to good yields under mild reaction conditions. Riluzole drug can be produced using the same procedure in moderate yields. In the experiment, the researchers used many compounds, for example, 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Safety of 6-Chlorobenzothiazol-2-ylamine)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Safety of 6-Chlorobenzothiazol-2-ylamineThe thiazole ring has been identified as a central feature of numerous natural products, perhaps the most famous example of which is epothilone.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

In 2017,Shankar, Bhookya; Jalapathi, Pochampally; Nagamani, Modhumpuram; Gandu, Bharath; Kudle, Karunakar Rao published 《Synthesis, anti-microbial activity, and cytotoxicity of novel 1-[5-[6-[(2-benzoylbenzofuran-5-yl)methyl]-2-oxo-2H-chromen-3-yl]thiazol-2-yl]urea derivatives》.Monatshefte fuer Chemie published the findings.Reference of 5-Bromothiazol-2-amine The information in the text is summarized as follows:

A series of novel 1-[5-[6-[(2-benzoylbenzofuran-5-yl)methyl]-2-oxo-2H-chromen-3-yl]thiazol-2-yl]urea derivatives I (NR2 = C6H4N, morpholino, cyclohexylamino, etc.) were synthesized by the reaction of 3-(2-aminothiazol-5-yl)-6-[(2-benzoylbenzofuran-5-yl)methyl]-2H-chromen-2-one with various substituted amines and triphosgene, in the presence of a base. The synthesized compounds were evaluated for their anti-microbial activity and cytotoxicity. Most of the compounds exhibited promising anti-microbial activity against the selected Gram-pos. and Gram-neg. bacterial strains at MIC values ranging from 0.071 to 0.199 μM and fungal pathogen was moderate to be good. The in vitro cytotoxicity testing of the title compounds was performed against cervical cancer (HeLa) cell lines. In the preliminary MTT cytotoxicity studies, the results have shown that few synthesized compounds which exhibit a significant cytotoxicity at microliter concentration and were found to be non-toxic with IC50 values ranging from 49.322/15 to 52.715/15 mm3. The experimental part of the paper was very detailed, including the reaction process of 5-Bromothiazol-2-amine(cas: 3034-22-8Reference of 5-Bromothiazol-2-amine)

5-Bromothiazol-2-amine(cas: 3034-22-8) belongs to anime. Acylation is one of the most important reactions of primary and secondary amines; a hydrogen atom is replaced by an acyl group (a group derived from an acid, such as RCOOH or RSO3H, by removal of ―OH, such as RC(=O)―, RS(O)2―, and so on). Reagents may be acid chlorides (RCOC1, RSO2C1), anhydrides ((RCO)2O), or even esters (RCOOR′); the products are amides of the corresponding acids.Reference of 5-Bromothiazol-2-amine

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

In 2018,Jo, Minmi; Won, Sun-Woo; Lee, Dong Guk; Jung, Jae-Kyung; Kim, Sunhong; Kwak, Young-Shin published 《An efficient synthetic protocol for amide derivatives of Boc-2-aminoisobutyrate》.Archives of Pharmacal Research published the findings.Recommanded Product: 5-Bromothiazol-2-amine The information in the text is summarized as follows:

Aminoisobutyric acid (AIB) is an important building block widely incorporated by medicinal chemists in mol. design. Owing to the steric challenge, elaborating AIB’s carboxylic acid using conventional amidation protocols is often problematic. We discovered that an amidation protocol utilizing Me Boc-aminoisobutyrate (Boc = tert-butoxycarbonyl) and magnesium amidates of various reactivities produces the corresponding amide derivatives in good to excellent yields. In the experimental materials used by the author, we found 5-Bromothiazol-2-amine(cas: 3034-22-8Recommanded Product: 5-Bromothiazol-2-amine)

5-Bromothiazol-2-amine(cas: 3034-22-8) belongs to anime. Nitrous acid converts secondary amines (aliphatic or aromatic) to N-nitroso compounds (nitrosamines): R2NH + HNO2 → R2N―NO. Some nitrosamines are potent cancer-inducing substances, and their possible formation is a serious consideration when nitrites, which are salts of nitrous acid, are present in foods or pharmaceutical preparations. Tertiary amines give rise to nitrosamines more slowly; an alkyl group is eliminated as an aldehyde or ketone, along with nitrous oxide, N2O.Recommanded Product: 5-Bromothiazol-2-amine

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

《Discovery of nitazoxanide-based derivatives as autophagy activators for the treatment of Alzheimer’s disease》 was written by Li, Xiaokang; Lu, Jian; Xu, Yixiang; Wang, Jiaying; Qiu, Xiaoxia; Fan, Lei; Li, Baoli; Liu, Wenwen; Mao, Fei; Zhu, Jin; Shen, Xu; Li, Jian. Category: thiazole And the article was included in Acta Pharmaceutica Sinica B in 2020. The article conveys some information:

The efficacy and discovery of new chem. entities, two series of NTZ-based derivatives I [R1 = H, SMe, SO2Me, etc.; R2 = 2-OH, 3-OH, 4-OH, 2-OAc] and II [R3 = Me, cyclohexyl, 2-MeC6H4, etc.] were designed, synthesized and evaluated as autophagy activator against AD. All compounds I and II were screened by the inhibition of phosphorylation of p70S6K, which was the direct substrate of mammalian target of rapamycin (mTOR) and its phosphorylation level could reflect the mTOR-dependent autophagy level. Among these analogs, compound II [R3 = 3-OAc] exhibited excellent potency in promoting β-amyloid (Aβ) clearance, inhibiting tau phosphorylation, as well as stimulating autophagy both in vitro and in vivo. Compound II [R3 = 3-OAc] could effectively improved the memory and cognitive impairments in APP/PS1 transgenic AD model mice. These results demonstrated that II [R3 = 3-OAc] was a potential candidate for the treatment of AD. In the experiment, the researchers used 5-Bromothiazol-2-amine(cas: 3034-22-8Category: thiazole)

5-Bromothiazol-2-amine(cas: 3034-22-8) belongs to anime. Amines can be classified according to the nature and number of substituents on nitrogen. Aliphatic amines contain only H and alkyl substituents. Aromatic amines have the nitrogen atom connected to an aromatic ring.Important amines include amino acids, biogenic amines, trimethylamine, and aniline. Inorganic derivatives of ammonia are also called amines, such as monochloramine (NClH2).Category: thiazole

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Related Products of 95-24-9On October 15, 2020 ,《Synthesis, characterization, pharmacological evaluation and molecular docking studies of benzothiazole azo derivatives》 was published in Journal of Molecular Structure. The article was written by Harisha, S.; Keshavayya, Jathi; Prasanna, S. M.; Joy Hoskeri, H.. The article contains the following contents:

A series of novel benzothiazole based azo dyes were synthesized and fully characterized by using different anal. techniques. The antioxidant activity of synthesized azo dyes was studied with the DPPH, hydrogen peroxide, metal chelating and nitric oxide radical methods and compared with the known antioxidant ascorbic acid. Further, the anticancer properties of synthesized azo dyes were carried out against breast cancer (MCF-7) cell lines by MTT assay and results revealed that the synthesized compounds exhibited good anticancer property in micro-molar range. Addnl., the anti-inflammatory activities of target compounds were also investigated by protein denaturation method and were found to have effective anti-inflammatory property. In order to predict the binding modes and binding affinity of synthesized compounds, they were docked into the active sites of protein B-cell lymphoma-extra-large (Bcl-xL) to predict their anticancer property. The synthesized compounds were found to have good affinity for B-cell lymphoma-extra-large (Bcl-xL). A good correlation was found between in-silico docking anal. and in biol. screening of all synthesized azo dyes with less binding energies and higher inhibition constants value against the target protein. The experimental process involved the reaction of 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9Related Products of 95-24-9)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Related Products of 95-24-9The thiazole ring has been identified as a central feature of numerous natural products, perhaps the most famous example of which is epothilone.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Mor, Satbir; Khatri, Mohini; Punia, Ravinder; Kumar, Deepak; Jindal, Deepak Kumar; Basu, Biswarup; Jakhar, Komal published their research in Journal of Molecular Structure on December 5 ,2022. The article was titled 《Synthesis and in vitro anticancer evaluation of 8b-hydroxy-1-(6-substitutedbenzo[d]thiazol-2-yl)-3-(3-substitutedphenyl)-1,8b-dihydroindeno[1,2-c]pyrazol-4(3aH)-ones》.SDS of cas: 95-24-9 The article contains the following contents:

A series of 1,8b-dihydroindeno[1,2-c]pyrazol-4(3aH)-ones I (R1 = Me, OMe, Br; R2 = H, Me, OMe, Cl, Br) has been efficiently syn.thesized on reaction of 2-(3-substitutedbenzoyl)-(1H)-indene-1,3(2H)-diones and 2-hydrazinylbenzo[d]thiazole/2-hydrazinyl-6-substituted benzo[d]thiazoles in presence of dry ethanol under reflux conditions in good yields. The synthesized hydroxypyrazolones were assayed for their anticancer activity against two human cancer cell lines (i) lung carcinoma (A549) and (ii) breast carcinoma (MCF7) using MTT assay. Among all the tested compounds, I (R1 = Me; R2 = Cl) and I (R1 = Br; R2 = H) with IC50 values in the range of 30.68 +/- 0.57μM to 34.87 +/- 0.76μM exhibited moderate inhibitory ability against both the tested cancer cell lines in comparison to the standard drug Doxorubicin (0.95 +/- 0.10μM for A549 and 1.09 +/- 0.11μM for MCF7). The above two compounds were further tested against normal human embryonic kidney cell line (HEK 293T) to examine their toxicity against normal cell lines. Selectivity Index (S.I.) of these compounds was calculated for both the cancer cell lines which revealed that these derivatives i.e. I (R1 = Me; R2 = Cl) (S.I = 2.82 for A549 and 2.90 for MCF7) and I (R1 = Br; R2 = H) (S.I = 2.78 for A549 and 2.95 for MCF7) are safer anticancer agents. In the experiment, the researchers used many compounds, for example, 6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9SDS of cas: 95-24-9)

6-Chlorobenzothiazol-2-ylamine(cas: 95-24-9) belongs to thiazoles. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.SDS of cas: 95-24-9The thiazole ring has been identified as a central feature of numerous natural products, perhaps the most famous example of which is epothilone.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica