Regulated preparation of celastrol-loaded nanoparticle by flash nanoprecipitation was written by Qi, Zhiyao;Qiu, Yuening;Zhong, Zilong;Wang, Junyou;Bian, Wei;Cohen Stuart, Martien A.;Wang, Mingwei. And the article was included in Journal of Drug Delivery Science and Technology in 2022.HPLC of Formula: 38215-36-0 The following contents are mentioned in the article:
Active components in Chinese medicine have shown great effects in treating various illness covering inflammation, obesity, diabetes and cancer. However, most of the active components, e.g. celastrol, suffer from the poor solubility and severe toxicity which limit their practical applications. One of the promising strategies is to load drugs in polymeric nanoparticles (NPs). While so far, most of the drug-loaded NPs were prepared by anti-solvent assembly or precipitation, which is a spontaneous and time-consuming process that provides thermodn. equilibrium NPs with typically low particle yield and drug loading content. Herein, we apply a kinetically controlled method, namely Flash Nanopptn. (FNP), to construct celastrol-loaded NPs. Specifically, solvent streams containing celastrol and biodegradable Dextran-b-PLGA are mixed with anti-solvent streams in a multi-inlet vortex mixer. The fast mixing and co-precipitation provide NPs with well-defined particle radius (80-160 nm, PDI <0.2) and structure, high celastrol loading content (11-63%) and tunable release as well. The regulation is achieved by manipulating the drug concentration and stream velocity (defined as Reynolds number). Moreover, the obtained NPs display efficient inhibition of A549 lung cancer cells, whereas notably reduced cytotoxicity to HL-7702 normal liver cell lines. Our study validates advantages of FNP method on preparing Chinese medicine drug-loaded NPs, and the achieved regulation on particle properties show great potential for boosting the application of Chinese medicine active components. This study involved multiple reactions and reactants, such as 3-(Benzo[d]thiazol-2-yl)-7-(diethylamino)-2H-chromen-2-one (cas: 38215-36-0HPLC of Formula: 38215-36-0).
3-(Benzo[d]thiazol-2-yl)-7-(diethylamino)-2H-chromen-2-one (cas: 38215-36-0) belongs to thiazole derivatives. The thiazole ring is notable as a component of the vitamin thiamine (B1). The pyridine-type nitrogen in the thiazole ring deactivates the ring for electrophilic substitution reactions, which is further reduced in acid due to protonation of the thiazole ring.HPLC of Formula: 38215-36-0
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica