Development of small-molecule-induced fibroblast expansion technologies was written by Sidal, Humeyra;Colakoglu Erkan, Pinar;Uslu, Merve;Kocabas, Fatih. And the article was included in Journal of Tissue Engineering and Regenerative Medicine in 2020.Synthetic Route of C16H19BrN2OS The following contents are mentioned in the article:
Dermal fibroblasts are responsible from the production of extracellular matrix and take role in the closure of skin wounds. Dermal fibroblasts are major cells of origin in the generation of induced pluripotent stem cells (IPSCs) and are historically being used as feeder layer and biofiller in the restorative surgeries. ex vivo expansion of the dermal fibroblasts provides a suitable model to study skin biol. and to engineer bioartifical skins. Thus, development of efficient fibroblast expansion technologies gets outmost importance day by day. We sought to identify small mols. that induce ex vivo fibroblast expansion and understand their mechanisms. We analyzed the effect of 35 small mols., which are expected to target mol. pathways involving cellular quiescence. We have found that small mols., especially AS1949490 and SKF96365, increase human dermal fibroblast expansion of at least three different fibroblasts. Cell cycle anal. confirms that these small mols. allow cell cycle progression, as evident by increased percentage of cells in S-G2-M phase of cell cycle. They led to a lower profile of apoptotic or necrotic fibroblasts. Intriguingly, we have found that identified small mols. could also endogenously induce the expression of IPSC generation, collagen synthesis, and aging-related genes. Identified small mols. may contribute to the induction of collagen synthesis in the biofiller products, the development of fibroblast products with better aging profile, and the improvement of IPSC generation. This study involved multiple reactions and reactants, such as 2-(2-Imino-4,5,6,7-tetrahydrobenzothiazol-3-yl)-1-p-tolylethanone Hydrobromide (cas: 63208-82-2Synthetic Route of C16H19BrN2OS).
2-(2-Imino-4,5,6,7-tetrahydrobenzothiazol-3-yl)-1-p-tolylethanone Hydrobromide (cas: 63208-82-2) belongs to thiazole derivatives. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities.Various laboratory methods exist for the organic synthesis of thiazoles. For example, 2,4-dimethylthiazole is synthesized from thioacetamide and chloroacetone.Synthetic Route of C16H19BrN2OS
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica