JIB-04 induces cell apoptosis via activation of the p53/Bcl-2/caspase pathway in MHCC97H and HepG2 cells was written by Liao, Weiguo;Liu, Jie;Liu, Bin;Huang, Xiaojie;Yin, Yongxin;Cai, De;Li, Mingyi;Zhu, Runzhi. And the article was included in Oncology Reports in 2018.Safety of 2-(2-Imino-4,5,6,7-tetrahydrobenzothiazol-3-yl)-1-p-tolylethanone Hydrobromide The following contents are mentioned in the article:
JIB-04 is a structurally unique small mol., known to exhibit anticancer activity and to inhibit the growth of human lung cancer and prostate cancer cell lines. However, the anticancer effect of JIB-04 against human hepatic carcinoma, and its underlying mechanisms, are still unclear. In the present study, MHCC97H and HepG2 cells were employed to investigate the anticancer effects of JIB-04 on cell viability and apoptosis. Annexin V/PI staining, a CCK-8 assay and western blot anal. demonstrated that JIB-04 induced apoptosis in MHCC97H and HepG2 cells, which was evidenced by the expression of proapoptotic and apoptotic proteins including p53, Bak, Bax, caspase-3 and caspase-9. Subsequently, the expression trends of Bcl-2 and p53 were reversed after co-treatment with pifithrin-α (PFT-α, a p53 inhibitor). The results revealed that JIB-04 suppressed the cell viability of MHCC97H and HepG2 cells in a concentration-dependent manner. Meanwhile, it was also demonstrated that JIB-04 effectively triggered MHCC97H and HepG2 cell apoptosis by downregulating Bcl-2/Bax expression, and upregulating proapoptotic and apoptotic protein expression via the p53/Bcl2/caspase signaling pathway. JIB-04 had effects on the inhibition of cell viability and the induction of apoptosis in MHCC97H and HepG2 cells. The underlying mechanism of action of JIB-04 was associated with the p53/Bcl-2/caspase signaling pathway. This study involved multiple reactions and reactants, such as 2-(2-Imino-4,5,6,7-tetrahydrobenzothiazol-3-yl)-1-p-tolylethanone Hydrobromide (cas: 63208-82-2Safety of 2-(2-Imino-4,5,6,7-tetrahydrobenzothiazol-3-yl)-1-p-tolylethanone Hydrobromide).
2-(2-Imino-4,5,6,7-tetrahydrobenzothiazol-3-yl)-1-p-tolylethanone Hydrobromide (cas: 63208-82-2) belongs to thiazole derivatives. Thiazoles in peptides or their ability to bind proteins, DNA and RNA has led to many synthetic studies and new applications. The pyridine-type nitrogen in the thiazole ring deactivates the ring for electrophilic substitution reactions, which is further reduced in acid due to protonation of the thiazole ring.Safety of 2-(2-Imino-4,5,6,7-tetrahydrobenzothiazol-3-yl)-1-p-tolylethanone Hydrobromide
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica