Wang, Jing’s team published research in ACS Biomaterials Science & Engineering in 6 | CAS: 30931-67-0

Wang, Jing published the artcileLabel-free colorimetric detection of acid phosphatase and screening of its inhibitors based on biomimetic oxidase activity of MnO₂ nanosheets, Quality Control of 30931-67-0, the publication is ACS Biomaterials Science & Engineering (2020), 6(5), 3132-3138, database is CAplus and MEDLINE.

In this research, we attempted to develop a sensitive colorimetric sensing strategy for the detection of acid phosphatase (ACP) based on MnO₂ nanosheets and explored its applications in screening and evaluating inhibitors of ACP. The MnO₂ nanosheets exhibit intrinsic biomimetic oxidase activity, which can catalyze the oxidation of the colorless 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonate) diammonium salt (ABTS) into green oxidized ABTS (oxABTS). Upon the introduction of ACP, L-ascorbic acid-2-phosphate can be dephosphorylated to ascorbic acid, which arouses the disintegration of MnO₂ nanosheets into Mn²⁺ ions. This disintegration weakens the enzyme mimicking activity of the MnO₂ nanosheets, leading to the impediment of the oxidation of ABTS. Conversely, in the absence of ACP, the ABTS is rapidly oxidized by MnO₂, leading to a significant colorimetric signal change. The absorbance difference at 420 nm displayed a linear relationship with the concentration of ACP ranging from 0.075 to 0.45 mU·mL^-1, generating a detection limit of 0.046 mU·mL^-1. In the inhibition assays, this sensing platform provided simple detection for parathion-Me (PM), a representative inhibitor of ACP. The facile evaluation of the inhibitory effect of PM, including its IC₅₀ toward ACP, was also realized.

ACS Biomaterials Science & Engineering published new progress about 30931-67-0. 30931-67-0 belongs to thiazole, auxiliary class Salt,Hydrazine,Amine,Benzothiazole, name is Ammonium 2,2′-(hydrazine-1,2-diylidene)bis(3-ethyl-2,3-dihydrobenzo[d]thiazole-6-sulfonate), and the molecular formula is C5H6BNO2, Quality Control of 30931-67-0.

Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/thiazole,
Thiazole | chemical compound | Britannica