Yang, Jimin published the artcileSolid-Phase Synthesis of Phosphorothioate Oligonucleotides Using Sulfurization Byproducts for in Situ Capping, Category: thiazole, the publication is Journal of Organic Chemistry (2018), 83(19), 11577-11585, database is CAplus and MEDLINE.
Phosphorothioate (PS) oligonucleotides are manufactured via a solid-phase chain elongation process in which a four-reaction cycle consisting of detritylation, coupling, sulfurization, and failure sequence capping with Ac2O is repeated. In the capping step uncoupled sequences are acetylated at the 5′-OH to stop the chain growth and control the levels of deletion, or (n-1), impurities. Herein, we report that the byproducts of the commonly used sulfurization reagents react with the 5′-OH and cap the failure sequences. The standard Ac2O capping step can therefore be eliminated and this 3-reaction cycle process affords higher yield and higher or comparable overall purity compared to the conventional 4-reaction synthesis. This improvement results in reducing the number of reactions from â?0 to â?0 for synthesis of a typical length 20-mer oligonucleotide. For every kilogram of an oligonucleotide intermediate synthesized, > 500 L of reagents and organic solvents are saved and the E-factor is decreased to < 1500 from â?000.
Journal of Organic Chemistry published new progress about 1192027-04-5. 1192027-04-5 belongs to thiazole, auxiliary class Other Aliphatic Heterocyclic,Amine, name is N,N-Dimethyl-N’-(3-thioxo-3H-1,2,4-dithiazol-5-yl)formimidamide, and the molecular formula is C12H15ClO3, Category: thiazole.
Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/thiazole,
Thiazole | chemical compound | Britannica