Decreased de novo synthesis of proteoglycans in drug-induced renal cystic disease was written by Lelongt, Brigitte;Carone, Frank A.;Kanwar, Yashpal S.. And the article was included in Proceedings of the National Academy of Sciences of the United States of America in 1988.HPLC of Formula: 6318-74-7 This article mentions the following:
Cellular and extracellular (tubular basement membrane, TBM) alterations in the proteoglycans (PGs) of the rat renal tubules in diphenylthiazole-induced cystic disease were investigated. The PGs of normal and cystic kidneys were labeled with [35S]sulfate in an organ-perfusion system. Extracted cellular and TBM PGs were characterized by Sepharose CL-6B chromatog. before or after treatment with heparitinase (degrades heparan sulfate) or chondroitinase ABC (degrades chondroitin sulfate). Total radioactivities in cellular, TBM, and medium fractions of cystic kidneys were reduced by factors of 9, 7, and 3, resp. The PGs obtained from cystic and normal kidneys had similar profiles, namely, two peaks of radioactivity with Kav values of 0.26 (Mr = 130,000-150,000) and 0.40 (Mr = 50,000-55,000). The peaks had variable proportions of radioactivity for cellular and TBM fractions. Besides heparan sulfate, an addnl. 15-20% of chondroitin sulfate was synthesized in all three fractions obtained from cystic kidneys. The PGs synthesized by cystic kidneys had lower charge-d. characteristics as compared to controls by DEAE-Sepharose chromatog. The medium fractions contained mostly glycosaminoglycan chains of heparan sulfate. Autoradiograms of tissue samples revealed ≈50% and ≈60% decreases of grain densities over the cellular and TBM compartments, resp. This decrease in de novo PG synthesis may have some relationship in the pathogenesis of polycystic kidney disease. In the experiment, the researchers used many compounds, for example, 4,5-Diphenylthiazol-2-amine (cas: 6318-74-7HPLC of Formula: 6318-74-7).
4,5-Diphenylthiazol-2-amine (cas: 6318-74-7) belongs to thiazole derivatives. Thiazoles are a class of five-membered rings containing nitrogen and sulfur with excellent antitumor, antiviral and antibiotic activities. The nitrogen in thiazole is sp2 hybridized and the lone pair of electrons localized on the nitrogen is less reactive due to increased aromatic character and decreased basicity. It is protonated and alkylated/acylated at nitrogen forming hydrochloride and quaternary thiazolium salt.HPLC of Formula: 6318-74-7
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica