Isolation and partial characterization of a peptide derived from the luciferin binding site of firefly luciferase was written by Lee, Reiko T.;McElroy, W. D.. And the article was included in Archives of Biochemistry and Biophysics in 1971.Quality Control of 6-Chlorobenzo[d]thiazole-2-carbonitrile This article mentions the following:
2-Cyano-6-chlorobenzothiazole (I), a substrate analog of firefly luciferase, inactivates this enzyme slowly at pH 8 to 鈭?0% of original activity without affecting free sulfhydryl groups. The inactivated luciferase contained 1.5-2.0 moles of I per 100,000 daltons of luciferase. It is believed that the benzothiazole derivatives are incorporated at the luciferin binding sites. Tryptic digest of the inactivated luciferase and subsequent electrophoresis yielded a fluorescent peptide containing benzothiazole derivative The peptide contained pyroglutamic acid (pyroGlu) at the N-terminal end. The sequence of the peptide was established tentatively to be: pyroGlu-X-Gly-Ala-Val-(Asp)-Ile-Leu where X is an amino acid (possibly Tyr) to which the benzothiazole derivative is attached. In the experiment, the researchers used many compounds, for example, 6-Chlorobenzo[d]thiazole-2-carbonitrile (cas: 26649-59-2Quality Control of 6-Chlorobenzo[d]thiazole-2-carbonitrile).
6-Chlorobenzo[d]thiazole-2-carbonitrile (cas: 26649-59-2) belongs to thiazole derivatives. Thiazole rings are planar and aromatic. Thiazoles are characterized by larger pi-electron delocalization than the corresponding oxazoles and have therefore greater aromaticity. Thiazole is a versatile building block for the construction and lead generation of new drug discoveries. Numerous diazole-based compounds are in clinical use as anticancer, antileukemic, antiinflammatory, antiviral, antifungal, antirheumatic, immunomodulator, and antiparasitic agents.Quality Control of 6-Chlorobenzo[d]thiazole-2-carbonitrile
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica