Reference of 1477-42-5, An article , which mentions 1477-42-5, molecular formula is C8H8N2S. The compound – 4-Methylbenzo[d]thiazol-2-amine played an important role in people’s production and life.
Capture-Tag-Release: A Strategy for Small Molecule Labeling of Native Enzymes
A strategy for labeling native enzymes in a manner that preserves their activity is reported: capture?tag?release (CTR). Key to this approach is the small molecule CTR probe that contains an enzyme inhibitor, benzophenone crosslinker, and aryl phosphine ester. After UV-derived capture of the enzyme, addition of an azide-containing tag triggers a Staudinger ligation that labels the enzyme. A further consequence of the Staudinger ligation is fragmentation of the CTR probe, thus releasing the inhibitor and restoring enzymatic activity. As a proof-of-principle, the CTR strategy was applied to the hydrolase beta-galactosidase. The enzyme was efficiently labeled with biotin, and the kinetic data for the biotinylated enzyme were comparable to those for unlabeled beta-galactosidase. The CTR probe exhibits excellent targeting specificity, as it selectively labeled beta-galactosidase in a complex protein mixture.
I hope this article can help some friends in scientific research. I am very proud of our efforts over the past few months and hope to 1477-42-5, help many people in the next few years., Reference of 1477-42-5
Reference：
Thiazole | C3H9566NS – PubChem,
Thiazole | chemical compound | Britannica