Kiehl, Isis G. A.;Riccetto, Eduardo;Salustiano, Ana C. C.;Ossick, Marina V.;Ferrari, Karen L.;Assalin, Heloisa B.;Ikari, Osamu;Reis, Leonardo O. published 《Boosting bladder cancer treatment by intravesical nitazoxanide and bacillus calmette-guerin association》 in 2021. The article was appeared in 《World Journal of Urology》. They have made some progress in their research.COA of Formula: C12H9N3O5S The article mentions the following:
Abstract: Purpose: Nitazoxanide (NTZ) has shown a promising antitumoral effect, the current study compared the anti-neoplastic effects of intravesical NTZ and BCG plus NTZ in NMIBC animal model. Methods: 30 rats, Fisher 344 were instilled with 4 intravesical doses of 1.5 mg/kg of N-methyl-N-nitrosourea (MNU) every 15 days for BC induction. The animals were divided into 3 groups (Group BCG 106 UFC -1 mg of BCG; Group NTZ -300 mg/kg of NTZ; Group NTZ + BCG -simultaneous treatment of BCG and NTZ) and received weekly intravesical treatment for 6 consecutive weeks. Animals were submitted to ultrasound imaging and euthanasia, their bladders were collected and histopathol., immunohistochem. tests (ki67 e c-Myc) and Western Blotting (PI3K, mTOR, and p-4E-BP) were performed. Results: Histopathol. tests showed 66.67%, 62.5% and 37.5% incidence of BC in animals treated with BCG, NTZ, and NTZ + BCG, resp. Nuclear positivity for ki-67 in BC animals were 12.4%, 13.2% and 8.8% in BCG, NTZ and NTZ + BCG group, resp. Between animals with carcinoma, c-Myc strong pos. was 40.10% in NTZ, 32.2% in BCG and 19.90% in the NTZ + BCG group. Blotting has shown mTOR (p =0.0473) and PI3K inhibition (p = 0.0349) in the presence of BCG, added to 4-EBP inhibition in the presence of NTZ. Conclusions: Results show the possible synergy between the gold standard BC treatment BCG and NTZ, in which multiple targets inhibition such as c-Myc and downstream mTOR, p-4E-BP and PI3K might play a role. The experimental procedure involved many compounds, such as 2-((5-Nitrothiazol-2-yl)carbamoyl)phenyl acetate (cas: 55981-09-4) .
2-((5-Nitrothiazol-2-yl)carbamoyl)phenyl acetate(cas: 55981-09-4) has been used: to test its anti-viral activity against chikungunya virus as an antiprotozoal agent to test its effect on cell viability in various cancer cell lines; to test its effect on human cytomegalovirus (HCMV) infected human fibroblast HFF cellsCOA of Formula: C12H9N3O5S
Reference:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica