Cheng, Gang; Pan, Jing; Podsiadly, Radoslaw; Zielonka, Jacek; Garces, Alexander M.; Dias Duarte Machado, Luiz Gabriel; Bennett, Brian; McAllister, Donna; Dwinell, Michael B.; You, Ming; Kalyanaraman, Balaraman published the artcile< Increased formation of reactive oxygen species during tumor growth: Ex vivo low-temperature EPR and in vivo bioluminescence analyses>, Computed Properties of 2591-17-5, the main research area is ROS tumor growth bioluminescence analysis boronate proluciferin probe ESR; Bioluminescence; EPR; Mitochondria; Oxidants; Tumor growth.
Previous studies have shown that reactive oxygen species (ROS) such as superoxide or hydrogen peroxide generated at low levels can exert a tumor-promoting role via a redox-signaling mechanism. Reports also suggest that both tumorigenesis and tumor growth are associated with enhanced ROS formation. However, whether ROS levels or ROS-derived oxidative marker levels increase during tumor growth remains unknown. In this study, in vivo bioluminescence imaging with a boronate-based pro-luciferin probe was used to assess ROS formation. Addnl., probe-free cryogenic ESR was used to quantify a characteristic aconitase [3Fe4S]+ center that arises in the tumor tissue of mouse xenografts from the reaction of the native [4Fe4S]2+ cluster with superoxide. Results indicated that tumor growth is accompanied by increased ROS formation, and revealed differences in oxidant formation in the inner and outer sections of tumor tissue, resp., demonstrating redox heterogeneity. Studies using luciferin and pro-luciferin probes enabled the assessment of tumor size, ROS formation, and bioenergetic status (e.g., ATP) in luciferase-transfected mice tumor xenografts. Probe-free ex vivo low-temperature ESR can also be translated to clin. studies.
Free Radical Biology & Medicine published new progress about Animal cell line (H2030). 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Computed Properties of 2591-17-5.
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica