Yao, Zi; Zhang, Brendan S.; Steinhardt, Rachel C.; Mills, Jeremy H.; Prescher, Jennifer A. published the artcile< Multicomponent Bioluminescence Imaging with a π-Extended Luciferin>, COA of Formula: C11H8N2O3S2, the main research area is multicomponent bioluminescence imaging luciferin.
Bioluminescence imaging with luciferase-luciferin pairs is commonly used for monitoring biol. processes in cells and whole organisms. Traditional bioluminescent probes are limited in scope, though, as they cannot be easily distinguished in biol. environments, precluding efforts to visualize multicellular processes. Addnl., many luciferase-luciferin pairs emit light that is poorly tissue penetrant, hindering efforts to visualize targets in deep tissues. To address these issues, the authors synthesized a set of π-extended luciferins that were predicted to be red shifted luminophores. The scaffolds were designed to be rotationally labile such that they produced light only when paired with luciferases capable of enforcing planarity. A luciferin comprising an intramol. “”lock”” was identified as a viable light-emitting probe. Native luciferases were unable to efficiently process the analog, but a complementary luciferase was identified via Rosetta-guided enzyme design. The unique enzyme-substrate pair is red shifted compared to known bioluminescent tools. The probe set is also orthogonal to other luciferase-luciferin probes and can be used for multicomponent imaging. Four substrate-resolved luciferases were imaged in a single session. Collectively, this work provides the first example of Rosetta-guided design in engineering bioluminescent tools and expands the scope of orthogonal imaging probes.
Journal of the American Chemical Society published new progress about Bioluminescent imaging. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, COA of Formula: C11H8N2O3S2.
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica