Nakajima, Kanako; Hamada, Kazuko; Ito, Ryoga; Yoshida, Yukina; Sutherland, Kenneth; Ishikawa, Masayori; Ozaki, Michitaka; Shirato, Hiroki; Hamada, Toshiyuki published the artcile< Stability of -luciferin for bioluminescence to detect gene expression in freely moving mice for long durations>, Product Details of C11H8N2O3S2, the main research area is Dluciferin L luciferin antiobesity agent obesity cancer; Period1; circadian rhythm; in vivo imaging; luciferin.
Circadian disturbance of clock gene expression is a risk factor for diseases such as obesity, cancer, and sleep disorders. To study these diseases, it is necessary to monitor and analyze the expression rhythm of clock genes in the whole body for a long duration. The bioluminescent reporter enzyme firefly luciferase and its substrate -luciferin have been used to generate optical signals from tissues in vivo with high sensitivity. However, little information is known about the stability of -luciferin to detect gene expression in living animals for a long duration. In the present study, we examined the stability of a luciferin solution over 21 days. -Luciferin, which is synthesized using racemization of -luciferin, was at high concentrations after 21 days. In addition, we showed that bioluminescence of Period1 (Per1) expression in the liver was significantly decreased compared with the day 1 solution, although locomotor activity rhythm was not affected. These results showed that -luciferin should be applied to the mouse within, at most, 7 days to detect bioluminescence of Per1 gene expression rhythm in vivo.
Luminescence published new progress about Acyl-CoA-binding proteins Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Product Details of C11H8N2O3S2.
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica