Zielonka, Jacek; Podsiadly, Radoslaw; Zielonka, Monika; Hardy, Micael; Kalyanaraman, Balaraman published the artcile< On the use of peroxy-caged luciferin (PCL-1) probe for bioluminescent detection of inflammatory oxidants in vitro and in vivo - Identification of reaction intermediates and oxidant-specific minor products>, HPLC of Formula: 115144-35-9, the main research area is inflammatory oxidant bioluminescence probe peroxy caged luciferin; Bioluminescence; Boronate probes; Luciferin; Spin traps.
Peroxy-caged luciferin (PCL-1)(I) probe was first used to image hydrogen peroxide in living systems (Van de Bittner et al., 2010 [9]). Recently this probe was shown to react with peroxynitrite more potently than with hydrogen peroxide (Sieracki et al., 2013 [11]) and was suggested to be a more suitable probe for detecting peroxynitrite under in vivo conditions. In this work, we investigated in detail the products formed from the reaction between PCL-1 and hydrogen peroxide, hypochlorite, and peroxynitrite. HPLC anal. showed that hydrogen peroxide reacts slowly with PCL-1, forming luciferin as the only product. Hypochlorite reaction with PCL-1 yielded significantly less luciferin, as hypochlorite oxidized luciferin to form a chlorinated luciferin. Reaction between PCL-1 and peroxynitrite consists of a major and minor pathway. The major pathway results in luciferin and the minor pathway produces a radical-mediated nitrated luciferin. Radical intermediate was characterized by spin trapping. We conclude that monitoring of chlorinated and nitrated products in addition to bioluminescence in vivo will help identify the nature of oxidant responsible for bioluminescence derived from PCL-1.
Free Radical Biology & Medicine published new progress about Bioluminescence. 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, HPLC of Formula: 115144-35-9.
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica