Kagiya, Go; Sato, Ayaka; Ogawa, Ryohei; Hatashita, Masanori; Kato, Mana; Kubo, Makoto; Kojima, Fumiaki; Kawakami, Fumitaka; Nishimura, Yukari; Abe, Naoya; Hyodo, Fuminori published the artcile< Real-time visualization of intratumoral necrosis using split-luciferase reconstitution by protein trans-splicing>, Quality Control of 2591-17-5, the main research area is intratumoral necrosis luciferase protein splicing; bioluminescent imaging; cell death; extein; intein; necrosis; necrosis imaging reporter; protein trans-splicing; split-luciferase reconstitution.
Necrosis, a form of cell death, occurs not only with the development of various diseases but also with a tumor tissue response to cancer treatment. Therefore, pursuing progress for cancer therapy through induction of necrosis may be one of the most effective approaches for cancer eradication. We herein describe the development of a real-time imaging system to visualize intratumoral necrosis. The system is composed of two types of cells expressing either one of two necrosis imaging reporters that consist of a DnaE intein sequence linking to one of two split-luciferase fragments. When necrosis occurs in a tumor composed of both of the cells, the two types of leaked reporters can reconstitute the enzymic activity as a result of protein trans-splicing and thereby emit bioluminescence in the presence of the substrate. This system, which was constructed with shrimp-derived luciferase, allowed in vitro imaging of necrosis. We further confirmed real-time imaging of intratumoral necrosis caused by phys. or chem. tissue disruption, validating its application in in vivo necrosis imaging. Thus, the constructed imaging system could be a powerful tool for the optimization of the therapeutic condition for cancer therapy and for the evaluation of novel anticancer drugs targeting necrosis.
Molecular Therapy–Oncolytics published new progress about Antitumor agents. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Quality Control of 2591-17-5.
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica