Electric Literature of C18H24N6O6S4On March 22, 2020, Thepchuay, Yanisa; Sonsa-ard, Thitaporn; Ratanawimarnwong, Nuanlaor; Auparakkitanon, Saranya; Sitanurak, Jirayu; Nacapricha, Duangjai published an article in Analytica Chimica Acta. The article was 《Paper-based colorimetric biosensor of blood alcohol with in-situ headspace separation of ethanol from whole blood》. The article mentions the following:
This work presents a novel development that exploits the concept of in-situ gas-separation together with a specific enzymic colorimetric detection to produce a portable biosensor called “”Blood Alc. Micro-pad”” for direct quantitation of ethanol in whole blood. The thin square device (25 mm × 25 mm × 1.8 mm) comprises two layers of patterned filter paper held together with a double-sided mounting tape with an 8-mm circular hole (the headspace). In operation, the reagent is deposited on one layer and covered with sticky tape. Then 8μL of a blood sample is dispensed onto the opposite layer and covered with sticky tape. Diffusion of ethanol across the 1.6 mm narrow headspace permits selective detection of ethanol by the enzymic reagents deposited on the opposite layer. This reagent zone contains alc. oxidase, horseradish peroxidase and 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, as the chromogenic reagent. The color intensity, measured from the recorded digital image, resulting from the enzymic assay of ethanol, correlates with the concentration of blood alc. The results obtained with spiked mice and sheep blood samples, using an external calibration in the range of 1-120 mg dL-1ethanol, gave recoveries of 93.2-104.4% (n = 12). The “”Blood Alc. Micro-pad”” gave good precision with %RSD <1 (50 mg dL-1 ethanol, n = 10) and limit of quantification (10SD of intercept/slope) of 11.56 mg dL-1. The method was successfully validated against a headspace gas chromatog.-mass spectrometric method. It has good potential for development as a simple and convenient blood alc. sensor for on-site testing. The results came from multiple reactions, including the reaction of ABTS Diammonium(cas: 30931-67-0Electric Literature of C18H24N6O6S4)
ABTS Diammonium(cas: 30931-67-0) belongs to anime. Large quantities of aliphatic amines are made synthetically. The most widely used industrial method is the reaction of alcohols with ammonia at a high temperature, catalyzed by metals or metal oxide catalysts (e.g., nickel or copper). Mixtures of primary, secondary, and tertiary amines are thereby produced.Electric Literature of C18H24N6O6S4
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica