《Metabolic characterization, antioxidant capacity, and In vitro antiurolithiatic potential of Achillea millefolium L.》 was published in Journal of Young Pharmacists in 2021. These research results belong to Kain, Dolly; Kumar, Suresh; Vandana; Suryavanshi, Amrita; Arya, Atul. Application In Synthesis of ABTS Diammonium The article mentions the following:
The current study investigated the antioxidant and antiurolithiatic potential of A. millefolium along with its metabolic characterization. A. millefolium has been collected at vegetative (V) and mature (Inflorescence (I) and Stem and Leaves (S+L)) stages and extracted in ethanol. Antioxidant potential has been determined by total phenolic content, total flavonoid content, ferric reducing antioxidant potential, total antioxidants through phosphomolybdate assay and free radical scavenging assays (1,1-diphenyl-2-picrylhydrazyl) and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid). In vitro antiurolithiatic activity is determined by turbidity changes in artificial urine, nucleation assay, and aggregation assay. Fourier transform-IR spectroscopy and gas chromatog.-mass spectrometry has been used to characterize the active metabolites of plant A. millefolium. Maximum antioxidant activity was observed in Inflorescence with values 76.58 mg of GAE/g of extract, 18.82 mg of RE/g of extract, 199.799 mg of AAE/g of extract and 327.95 mg of AAE/g of extract for TPC, TFC, FRAP and total antioxidants through phosphomolybdate assay resp. Maximum radical scavenging activity was also observed in inflorescence with 86.3% and 69.655 % inhibition against DPPH and ABTS free radicals at 1000μg/mL concentration resp. Maximum in vitro antiurolithiatic potential of plant A. millefolium observed in inflorescence i.e. 80 %, 41.84% and 63.41 % inhibition at 1000μg/mL concentration for turbidity changes in artificial urine method, nucleation assay and aggregation assay resp. FT- IR and GCMS of inflorescence of A. millefolium confirmed the presence of major functional groups and active metabolic compounds resp. Inflorescence of plant A. millefolium is an excellent source of natural antioxidants with significant antiurolithiatic potential. In the experiment, the researchers used many compounds, for example, ABTS Diammonium(cas: 30931-67-0Application In Synthesis of ABTS Diammonium)
ABTS Diammonium(cas: 30931-67-0) belongs to anime. To avoid the problem of multiple alkylation, methods have been devised for “blocking” substitution so that only one alkyl group is introduced. The Gabriel synthesis is one such method; it utilizes phthalimide, C6H4(CO)2NH, whose one acidic hydrogen atom has been removed upon the addition of a base such as KOH to form a salt.Application In Synthesis of ABTS Diammonium
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica