Yin, Qiang; Zhou, Gang; Peng, Can; Zhang, Yinliang; Kues, Ursula; Liu, Juanjuan; Xiao, Yazhong; Fang, Zemin published their research in AMB Express on December 31 ,2019. The article was titled 《The first fungal laccase with an alkaline pH optimum obtained by directed evolution and its application in indigo dye decolorization》.Related Products of 30931-67-0 The article contains the following contents:
Engineering of fungal laccases with optimum catalytic activity at alk. pH has been a long-lasting challenge. In this study, a mutant library containing 3000 clones was obtained by error-prone PCR to adapt the optimum pH of a fungal laccase Lcc9 from the basidiomycete Coprinopsis cinerea. After three rounds of functional screening, a mutant with three amino acid changes (E116K, N229D, I393T) named PIE5 was selected. PIE5 showed an optimum pH of 8.5 and 8.0 against guaiacol and 2,6-DMP when expressed in Pichia pastoris, representing the first fungal laccase that possesses an optimum pH at an alk. condition. Site directed mutagenesis disclosed that N229D contributed the most to the optimum pH increment. A single N229D mutation caused an increase in optimum pH by 1.5 units. When used in indigo dye decolorization, PIE5 efficiently decolorized 87.1 ± 1.1% and 90.9 ± 0.3% indigo dye at the optimum conditions of pH 7.0-7.5 and 60°C, and with either Me 3,5-dimethoxy-4-hydroxybenzoate or 2,2′-azino-bis(3-ethylbenzothazoline-6-sulfonate) as the mediator. In comparison, the com. available fungal laccase TvLac from Trametes villosa decolorized 84.3 ± 1.8% of indigo dye under its optimum conditions (opt. pH 5.0 and 60°C). The properties of an alk.-dependent activity and the high indigo dye decolorization ability (1.3-fold better than the parental Lcc9) make the new fungal laccase PIE5 an alternative for specific industrial applications. After reading the article, we found that the author used ABTS Diammonium(cas: 30931-67-0Related Products of 30931-67-0)
ABTS Diammonium(cas: 30931-67-0) belongs to anime. Amine, any member of a family of nitrogen-containing organic compounds that is derived, either in principle or in practice, from ammonia (NH3). Naturally occurring amines include the alkaloids, which are present in certain plants; the catecholamine neurotransmitters (i.e., dopamine, epinephrine, and norepinephrine); and a local chemical mediator, histamine, that occurs in most animal tissues.Related Products of 30931-67-0
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica