On June 30, 2017, Hua, Jin published a patent.Recommanded Product: 64987-16-2 The title of the patent was Preparation method of immunoaffinity column (IAC) for detecting sulfonamide residue in animal-derived food. And the patent contained the following:
The title preparation method includes (1) preparing sulfonamide artificial antigens TS-BSA and SM2-BSA by sep. coupling tetramethylthiuram monosulfide (TS) antigen and sulfadimidine SM2 antigen with bovine serum albumin (BSA) and ovalbumin; (2) immunizing mouse, collecting B lymphocytes, fusing with myeloma cells to obtain hybridoma cells, and preparing Batroxobin toxin monoclonal antibody 5H4, NR3C2 monoclonal antibody 2B5 and PPBP monoclonal antibody 5C7 by in vivo induced ascites generation method; and (3) sep. coupling the monoclonal antibodies with cyanogen bromide-activated sepharose 4B, mixing at ratio of 1:1:1, and packing into a column to obtain composite immunoaffinity column (IAC). The composite IAC has good specific adsorption effect on at least 12 kinds of sulfonamides as follows: ulfaquinoxaline, sulfadiazine, sulfapyridine, sulfameter, sulfadimidine, sulfamethoxazole, sulfisoxazole, sulfadimethoxypyrimidine, sulfathiazole, slfamerazine free acid, sodium N-(6-chloropyridazin-3-yl)sulphanilamidate and sulfamonomethoxine. The inventive IAC has high coupling rate, strong specific adsorption and high column capacity and yield, and can be used for detecting the above sulfonamide residues in animal-derived food sensitively, rapidly and simply. The experimental process involved the reaction of Methyl 2-(2-aminothiazol-4-yl)acetate(cas: 64987-16-2).Recommanded Product: 64987-16-2
The Article related to immunoaffinity chromatog animal derived food sulfonamide feed additives spectrometry, Biochemical Methods: Chromatographic and other aspects.Recommanded Product: 64987-16-2
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica