Samei, Amit published the artcileSimultaneous estimation of related impurities of tizanidine hydrochloride in its active pharmaceutical ingredient by reversed-phase liquid chromatography, Recommanded Product: 5-Chlorobenzo[c][1,2,5]thiadiazol-4-amine, the publication is Asian Journal of Chemistry (2009), 21(1), 176-182, database is CAplus.
A simple method was developed for qual. and quant. estimation of tizanidine hydrochloride and its 5 structurally related impurities using a reversed-phase liquid chromatog. This method was validated as per ICH guidelines for ‘validation for anal. procedures’ Q2B. The liquid chromatog. was set up in UV detection mode at 310 nm. A separation was achieved between tizanidine and all 5 impurities in a gradient programming using YMC Pack Pro C18 (250 mm × 4.6 mm), 5 μ column at 1 mL/min flow rate. This method was established to be linear in a concentration range of (0.5-4.0 μg/mL) for all the components considered in this study. Limit of detection (LOD) and limit of quantification (LOQ) values were found to be (0.1-0.15 μg/mL) and (0.3-0.45 μg/mL) for tizanidine hydrochloride and its 5 related impurities, resp. Recoveries of all spiked impurities in tizanidine hydrochloride were in a range of (95-105%). Robustness and sample solution stability studies demonstrated the ruggedness of this method. This method is simple, sensitive and provides precisely accurate results.
Asian Journal of Chemistry published new progress about 30536-19-7. 30536-19-7 belongs to thiazole, auxiliary class Other Aromatic Heterocyclic,Chloride,Amine, name is 5-Chlorobenzo[c][1,2,5]thiadiazol-4-amine, and the molecular formula is C6H4ClN3S, Recommanded Product: 5-Chlorobenzo[c][1,2,5]thiadiazol-4-amine.
Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/thiazole,
Thiazole | chemical compound | Britannica