Selective Detection of 5-Formyl-2′-deoxyuridine, an Oxidative Lesion of Thymidine, in DNA by a Fluorogenic Reagent was written by Hirose, Wataru;Sato, Kousuke;Matsuda, Akira. And the article was included in Angewandte Chemie, International Edition in 2010.Formula: C9H10N2O2S This article mentions the following:
The authors report a new concept for the simple detection of 5-Formyl-2′-deoxyuridine (fodUrd) in damaged DNA with a fluorogenic reagent. The reagent 2-amino-4,5-dimethoxythiophenol (I) shows no fluorescence before reaction with the target fodUrd in DNA. However, upon the reaction of I with fodUrd, the formyl group at the 5-position of fodUrd is converted into a benzothiazol-2-yl group, which is directly conjugated with the uracil group. This ring system is similar to luciferin, which undergoes the luciferase reaction to produce luminescence. Thus, fodUrd in DNA could be detected directly by fluorescence measurement without enzymic hydrolysis of the target DNA to its corresponding nucleosides, HPLC separation, and anal. In the experiment, the researchers used many compounds, for example, 5,6-Dimethoxybenzo[d]thiazol-2-amine (cas: 6294-52-6Formula: C9H10N2O2S).
5,6-Dimethoxybenzo[d]thiazol-2-amine (cas: 6294-52-6) belongs to thiazole derivatives. Thiazole rings are planar and aromatic. Thiazoles are characterized by larger pi-electron delocalization than the corresponding oxazoles and have therefore greater aromaticity. Various laboratory methods exist for the organic synthesis of thiazoles. Prominent is the Hantzsch thiazole synthesis is a reaction between haloketones and thioamides.Formula: C9H10N2O2S
Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica