Category: 115144-35-9

Bellini, Michela; Riva, Benedetta; Tinelli, Veronica; Rizzuto, Maria Antonietta; Salvioni, Lucia; Colombo, Miriam; Mingozzi, Francesca; Visioli, Alberto; Marongiu, Laura; Frascotti, Gianni; Christodoulou, Michael S.; Passarella, Daniele; Prosperi, Davide; Fiandra, Luisa published the artcile< Engineered Ferritin Nanoparticles for the Bioluminescence Tracking of Nanodrug Delivery in Cancer>, Synthetic Route of 115144-35-9, the main research area is ferritin nanoparticle bioluminescence imaging cancer; apoferritin nanoparticles; luciferase/luciferin; nanomedicine; self-immolative linkers; tumor targeting.

The identification of a highly sensitive method to check the delivery of administered nanodrugs into the tumor cells is a crucial step of preclin. studies aimed to develop new nanoformulated cures, since it allows the real therapeutic potential of these devices to be forecast. In the present work, the ability of an H-ferritin (HFn) nanocage, already investigated as a powerful tool for cancer therapy thanks to its ability to actively interact with the transferrin receptor 1, to act as an efficient probe for the monitoring of nanodrug delivery to tumors is demonstrated. The final formulation is a bioluminescent nanoparticle, where the luciferin probe is conjugated on nanoparticle surface by means of a disulfide containing linker (Luc-linker@HFn) which is subjected to glutathione-induced cyclization in tumor cell cytoplasm. The prolonged imaging of luciferase+ tumor models, demonstrated by an in vitro and an in vivo approach, associated with the prolonged release of luciferin into cancer cells by disulfide bridge reduction, clearly indicates the high efficiency of Luc-linker@HFn for drug delivery to the tumor tissues.

Small published new progress about Apoferritins Role: NAN (Nanomaterial), THU (Therapeutic Use), BIOL (Biological Study), USES (Uses). 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, Synthetic Route of 115144-35-9.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Yu, Dou; Li, Gina; Lesniak, Maciej S.; Balyasnikova, Irina V. published the artcile< Intranasal delivery of therapeutic stem cells to glioblastoma in a mouse model>, Quality Control of 115144-35-9, the main research area is intranasal administration stem cell therapy glioblastoma.

The intrinsic tropism towards brain malignancies renders stem cells as promising carriers of therapeutic agents against malignant tumors. The delivery of therapeutic stem cells via the intranasal route is a recently discovered alternative strategy, with strong potential for clin. translation, due to its non-invasive nature compared to intracranial implantation or delivery via systemic routes. The lack of blood brain barrier further strengthens the therapeutic potential of stem cells undergoing intranasal brain entry. This article summarizes the essential techniques utilized in our studies and outlines the basic principles of intranasal strategy for stem cell delivery using a mouse model of intracranial glioma xenografts. We demonstrate the optimized procedures that generate consistent and reproducible results with specific predetermined exptl. parameters and offer guidelines for streamlined work flow that ensure efficient execution and reliable exptl. outcome. The article is designed to serve as a baseline for further exptl. customization based on hypothesis, stem cell types, or tumor specifics.

Journal of Visualized Experiments published new progress about Adenoviral vectors. 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, Quality Control of 115144-35-9.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Zielonka, Jacek; Podsiadly, Radoslaw; Zielonka, Monika; Hardy, Micael; Kalyanaraman, Balaraman published the artcile< On the use of peroxy-caged luciferin (PCL-1) probe for bioluminescent detection of inflammatory oxidants in vitro and in vivo - Identification of reaction intermediates and oxidant-specific minor products>, HPLC of Formula: 115144-35-9, the main research area is inflammatory oxidant bioluminescence probe peroxy caged luciferin; Bioluminescence; Boronate probes; Luciferin; Spin traps.

Peroxy-caged luciferin (PCL-1)(I) probe was first used to image hydrogen peroxide in living systems (Van de Bittner et al., 2010 [9]). Recently this probe was shown to react with peroxynitrite more potently than with hydrogen peroxide (Sieracki et al., 2013 [11]) and was suggested to be a more suitable probe for detecting peroxynitrite under in vivo conditions. In this work, we investigated in detail the products formed from the reaction between PCL-1 and hydrogen peroxide, hypochlorite, and peroxynitrite. HPLC anal. showed that hydrogen peroxide reacts slowly with PCL-1, forming luciferin as the only product. Hypochlorite reaction with PCL-1 yielded significantly less luciferin, as hypochlorite oxidized luciferin to form a chlorinated luciferin. Reaction between PCL-1 and peroxynitrite consists of a major and minor pathway. The major pathway results in luciferin and the minor pathway produces a radical-mediated nitrated luciferin. Radical intermediate was characterized by spin trapping. We conclude that monitoring of chlorinated and nitrated products in addition to bioluminescence in vivo will help identify the nature of oxidant responsible for bioluminescence derived from PCL-1.

Free Radical Biology & Medicine published new progress about Bioluminescence. 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, HPLC of Formula: 115144-35-9.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Verhoef, Lisette G. G. C.; Mattioli, Michela; Ricci, Fernanda; Li, Yao-Cheng; Wade, Mark published the artcile< Multiplex detection of protein-protein interactions using a next generation luciferase reporter>, Recommanded Product: Potassium (S)-2-(6-hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylate, the main research area is protein protein interactions assay luciferase reporter; Cancer; Interaction; Luciferase; MDM2; PPI; p53.

Cell-based assays of protein-protein interactions (PPIs) using split reporter proteins can be used to identify PPI agonists and antagonists. Split luciferase systems offer advantages over those that use split fluorescent proteins (FPs). This is since split luciferase offers a greater signal:noise ratio and, unlike split FPs, the PPI can be reversed upon small mol. treatment. While multiplexed PPI assays using luciferase have been reported, they suffer from low signal:noise and require fairly complex spectral deconvolution during anal. Furthermore, the luciferase enzymes used are large, which limits the range of PPIs that can be interrogated due to steric hindrance from the split luciferase fragments. Here, we report a multiplexed PPI assay based on split luciferases from Photinus pyralis (firefly luciferase, FLUC) and the deep-sea shrimp, Oplophorus gracilirostris (NanoLuc, NLUC). Specifically, we show that the binding of the p53 tumor suppressor to its two major neg. regulators, MDM2 and MDM4, can be simultaneously measured within the same sample, without the requirement for complex filters or deconvolution. We provide chem. and genetic validation of this system using MDM2-targeted small mols. and mutagenesis, resp. Combined with the superior signal:noise and smaller size of split NanoLuc, this multiplexed PPI assay format can be exploited to study the induction or disruption of pairwise interactions that are prominent in many cell signaling pathways.

Biochimica et Biophysica Acta, Molecular Cell Research published new progress about Bioassay. 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, Recommanded Product: Potassium (S)-2-(6-hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylate.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Wang, Ji-Quan; Pollok, Karen E.; Cai, Shanbao; Stantz, Keith M.; Hutchins, Gary D.; Zheng, Qi-Huang published the artcile< PET imaging and optical imaging with D-luciferin [11C]methyl ester and D-luciferin [11C]methyl ether of luciferase gene expression in tumor xenografts of living mice>, Computed Properties of 115144-35-9, the main research area is luciferin carbon 11 methylester methylether PET luciferase gene tumor; optical imaging luciferase gene expression tumor.

New carbon-11 labeled -luciferin analogs -luciferin [11C]methyl ester ([11C]LMEster, [11C]1) and -luciferin [11C]methyl ether ([11C]LMEther, [11C]2) were synthesized in 25-55% radiochem. yield. PET studies with [11C]LMEster and [11C]LMEther demonstrate a lower retention of the C-11 label at 45 min post-injection in luciferase expression tumor. Optical imaging with unlabeled substrate -luciferin and radiotracers [11C]LMEster and [11C]LMEther gave tumor luciferase images within a few minutes of photon counting.

Bioorganic & Medicinal Chemistry Letters published new progress about Animal gene, luc Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, Computed Properties of 115144-35-9.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Eiriksdottir, Emelia; Langel, Uelo; Rosenthal-Aizman, Katri published the artcile< An improved synthesis of releasable luciferin-CPP conjugates>, Product Details of C11H7KN2O3S2, the main research area is synthesis releasable luciferin cell penetrating peptide conjugate.

The authors have improved the synthesis of a previously published luciferin-linker, used in an assay enabling rapid real-time quantification of luciferin-CPP conjugate uptake and cytosolic cargo release. The authors also present the synthesis of a new luciferin-linker with the same conjugation ability. Both luciferin-linkers are now available via an efficient one-pot procedure.

Tetrahedron Letters published new progress about 115144-35-9. 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, Product Details of C11H7KN2O3S2.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Cheng, Lin; Su, Lantian; Tian, Xiaowen; Xia, Fan; Zhao, Chang; Yan, Wei; Shao, Zhenhua published the artcile< A pipeline to investigate the structures and signaling pathways of sphingosine 1-phosphate receptors>, Quality Control of 115144-35-9, the main research area is sphingosine 1 phosphate receptor structure signaling pathway investigation.

Lysophospholipids (LPLs) are bioactive lipids that include sphingosine 1-phosphate (S1P), lysophosphatidic acid, etc. S1P, a metabolic product of sphingolipids in the cell membrane, is one of the best-characterized LPLs that regulates a variety of cellular physiol. responses via signaling pathways mediated by sphingosine 1-phosphate receptors (S1PRs). This implicated that the S1P-S1PRs signaling system is a remarkable potential therapeutic target for disorders, including multiple sclerosis (MS), autoimmune disorders, cancer, inflammation, and even COVID-19. S1PRs, a small subset of the class A G-protein coupled receptor (GPCR) family, are composed of five subtypes: S1PR1, S1PR2, S1PR3, S1PR4, and S1PR5. The lack of detailed structural information, however, impedes the drug discovery targeting S1PRs. Here, we applied the cryo-electron microscopy method to solve the structure of the S1P-S1PRs complex, and elucidated the mechanism of activation, selective drug recognition, and G-protein coupling by using cell-based functional assays. Other lysophospholipid receptors (LPLRs) and GPCRs can also be studied using this strategy.

Journal of Visualized Experiments published new progress about Autoimmune disease. 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, Quality Control of 115144-35-9.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Liu, Pei-Han; Urban, Pawel L. published the artcile< Spontaneous luminescence color change in the firefly luciferase assay system>, Application of C11H7KN2O3S2, the main research area is luminescence color firefly luciferase; ATP assay; Color switching; Luciferase; Luminescence.

The temporal effects of luciferase reaction luminescence have only been discussed in the context of light intensity (flash vs. glow). However, alterations in the color of the light emitted over the luciferase reaction have not been reported. Here, the authors show a temporal change in the light color emitted during the reaction catalyzed by unmodified firefly luciferase when concentrations of one of the substrates, ATP, are gradually increased. The temporal color change from green to red occurs within the first few minutes of the luciferase reaction when an ATP-containing solution is either added or synthesized in situ with the aid of an autocatalytic reaction occurring simultaneously. This color change is not accompanied by pH changes. An anal. of the red and green channels demonstrates dissimilar kinetics, suggesting the coexistence of two or more temporally shifted luminescence pathways. The implications of these findings might improve dual-color biosensing/imaging protocols and influence the engineering of biophotonic systems.

Analytical Biochemistry published new progress about Color. 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, Application of C11H7KN2O3S2.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Van Zyl, Winschau F.; Deane, Shelly M.; Dicks, Leon M. T. published the artcile< In vivo bioluminescence imaging of the spatial and temporal colonization of lactobacillus plantarum 423 and enterococcus mundtii ST4SA in the intestinal tract of mice>, Formula: C11H7KN2O3S2, the main research area is intestinal tract Lactobacillus Enterococcus spatial temporal colonization bioluminescence imaging; Colonization; Enterococcus mundtii ST4SA; Gastrointestinal tract; In vivo bioluminescence imaging; Intestinal persistence; Lactic acid bacteria; Lactobacillus plantarum 423; Luciferase.

Background: Lactic acid bacteria (LAB) are major inhabitants and part of the normal microflora of the gastrointestinal tract (GIT) of humans and animals. Despite substantial evidence supporting the beneficial properties of LAB, only a few studies have addressed the migration and colonization of probiotic bacteria in the GIT. The reason for this is mostly due to the limitations, or lack of, efficient reporter systems. Here we describe the development and application of a non-invasive in vivo bioluminescence reporter system to study, in real-time, the spatial and temporal persistence of Lactobacillus plantarum 423 and Enterococcus mundtii ST4SA in the intestinal tract of mice. Results: This study reports on the application of the firefly luciferase gene (ffluc) from Photinus pyralis to develop luciferase-expressing L. plantarum 423 and E. mundtii ST4SA, using a Lactococcus lactis NICE system on a high copy number plasmid (pNZ8048) and strong constitutive lactate dehydrogenase gene promoters (Pldh and STldh). The reporter system was used for in vivo and ex vivo monitoring of both probiotic LAB strains in the GIT of mice after single and multiple oral administrations. Enterococcus mundtii ST4SA reached the large intestine 45 min after gavage, while L. plantarum 423 reached the cecum/colon after 90 min. Both strains predominantly colonized the cecum and colon after five consecutive daily administrations. Enterococcus mundtii ST4SA persisted in feces at higher numbers and for more days compared to L. plantarum 423. Conclusions: Our findings demonstrate the efficiency of a high-copy number vector, constitutive promoters and bioluminescence imaging to study the colonization and persistence of L. plantarum 423 and E. mundtii ST4SA in the murine GIT. The system allowed us to differentiate between intestinal transit times of the two strains in the digestive tract. This is the first report of bioluminescence imaging of a luciferase-expressing E. mundtii strain to study colonization dynamics in the murine model. The bioluminescence system developed in this study may be used to study the in vivo colonization dynamics of other probiotic LAB.

BMC Microbiology published new progress about Bioluminescent imaging. 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, Formula: C11H7KN2O3S2.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Li, Rifei; Zhu, Xinjie; Zhou, Peng; Qiao, Yuehua; Li, Yinqian; Xu, Yice; Shi, Xi published the artcile< Generation of a high-affinity nanobody against CD147 for tumor targeting and therapeutic efficacy through conjugating doxorubicin>, Category: thiazole, the main research area is CD147 tumor targeting conjugating doxorubicin anticancer agent breast cancer; CD147; DOX–11-1; nanobody; phage display; tumor targeting.

CD147, a glycosylated transmembrane protein in the Ig superfamily, is overexpressed on the surfaces of various tumor cells and promotes cancer cell proliferation, invasion, and metastasis. Nanobodies, characterized by small sizes, high affinities and specificities, and low immunogenicities, are promising diagnostic and therapeutic tools. However, there are few reports on nanobodies that specifically target CD147. In this work, a specific anti-CD147 nanobody has been successfully identified using phage display technol. The tumor target and antitumor effects have also been detected in different CD147-pos. tumors in in vitro and in vivo assays, resp. Meanwhile, it has a synergistic effect for inhibiting 4T1-bearing mice through conjugating doxorubicin. It may afford new strategies for cancer therapies.

Frontiers in Immunology published new progress about Antitumor agents. 115144-35-9 belongs to class thiazole, and the molecular formula is C11H7KN2O3S2, Category: thiazole.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica