Category: 2591-17-5

Jin, Minye; Glaeser, Alisa; Paez, Julieta I. published the artcile< Redox-triggerable firefly luciferin-bioinspired hydrogels as injectable and cell-encapsulating matrices>, Product Details of C11H8N2O3S2, the main research area is luciferin bioinspired hydrogel injectable cell encapsulating matrix.

Stimuli-responsive hydrogels are smart materials that respond to variations caused by external stimuli and that are currently exploited for biomedical applications such as biosensing, drug delivery and tissue engineering. The development of stimuli-responsive hydrogels with defined user control is relevant to realize materials with advanced properties. Recently, our group reported firefly luciferin-inspired hydrogel matrixes for 3D cell culture. This platform exhibited advantages like rapid gelation rate and tunability of mech. and biol. properties. However, this first mol. design did not allow fine control of the gelation onset, which restricts application as a cell-encapsulating matrice with injectable and processable properties. In this article, we endow the firefly luciferin-inspired hydrogels with redox-triggering capability, to overcome the limitations of the previous system and to widen its application range. We achieve this goal by introducing protected macromers as hydrogel polymeric precursors that can be activated in the presence of a mild reductant, to trigger gel formation in situ with a high degree of control. We demonstrate that the regulation of mol. parameters (e.g., structure of the protecting group, reductant type) and environmental parameters (e.g., pH, temperature) of the deprotection reaction can be exploited to modulate materials properties. This redox-triggerable system enables precise control over gelation onset and kinetics, thus facilitating its utilization as an injectable hydrogel without neg. impacting its cytocompatibility. Our findings expand the current toolkit of chem.-based stimuli-responsive hydrogels.

Polymer Chemistry published new progress about Biocompatibility, cytocompatibility. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Product Details of C11H8N2O3S2.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Morciano, Giampaolo; Imamura, Hiromi; Patergnani, Simone; Pedriali, Gaia; Giorgi, Carlotta; Pinton, Paolo published the artcile< Measurement of ATP concentrations in mitochondria of living cells using luminescence and fluorescence approaches>, Reference of 2591-17-5, the main research area is ATP; Bioluminescence; Biosensor; FRET; Fluorescence; Live cell imaging; Luciferase assay; Mitochondria.

Adenosine 5′-triphosphate (ATP) is the central metabolite in the energy metabolism of cells and is hydrolyzed to ADP and inorganic phosphate to provide free energy in various cellular processes. ATP also functions as an intracellular signaling mol. Thus, it is important to know the ATP concentration within cells to understand cellular activities. Here, we describe two methods to detect ATP concentrations in the cytoplasm and mitochondrial matrix using genetically encoded luminescent or fluorescent biosensors. These methods enable quant. investigation of ATP concentration dynamics in living cells, single cells and cell populations.

Methods in Cell Biology published new progress about 2591-17-5. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Reference of 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Ogawa, Haruhisa; Ono, Ryohei; Noguchi, Yoshifumi; Kitada, Nobuo; Saito-Moriya, Ryohei; Maki, Shojiro A.; Akiyama, Hidefumi; Itabashi, Hideyuki; Hiyama, Miyabi published the artcile< Absorption Spectra for Firefly Bioluminescence Substrate Analog: TokeOni in Various pH Solutions>, Reference of 2591-17-5, the main research area is AkaLumine TokeOni protonation dissociation constant UV spectrum.

AkaLumine hydrochloride, named TokeOni, is one of the firefly luciferin analogs, and its reaction with firefly luciferase produces near-IR (NIR) bioluminescence. Prior to studying the bioluminescence mechanism, basic knowledge about the chem. structures, electronic states, and absorption properties of TokeOni at various pH values of solution has to be acquired. In this paper, the absorption spectra for TokeOni and AkaLumine at pH 2-10 were measured. D. functional theory (DFT) calculations, time-dependent DFT calculations, and the vibrational analyses were carried out. The absorption spectra indicate that the chem. forms of TokeOni in solutions are same as those of AkaLumine. The peaks at pH 7-10 in the absorption spectra correspond to the excitation from the ground state of a carboxylate anion of AkaLumine, the peak at pH 2 corresponds to the excitation from the ground state of a carboxylate anion with an N-protonated thiazoline ring and N-protonated dimethylamino group of AkaLumine, and the peak at pH 4 corresponds to the excitation from the ground state of a carboxylate anion with an N-protonated thiazoline ring of AkaLumine.

Photochemistry and Photobiology published new progress about Bioluminescence. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Reference of 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Mitiouchkina, Tatiana; Mishin, Alexander S.; Somermeyer, Louisa Gonzalez; Markina, Nadezhda M.; Chepurnyh, Tatiana V.; Guglya, Elena B.; Karataeva, Tatiana A.; Palkina, Kseniia A.; Shakhova, Ekaterina S.; Fakhranurova, Liliia I.; Chekova, Sofia V.; Tsarkova, Aleksandra S.; Golubev, Yaroslav V.; Negrebetsky, Vadim V.; Dolgushin, Sergey A.; Shalaev, Pavel V.; Shlykov, Dmitry; Melnik, Olesya A.; Shipunova, Victoria O.; Deyev, Sergey M.; Bubyrev, Andrey I.; Pushin, Alexander S.; Choob, Vladimir V.; Dolgov, Sergey V.; Kondrashov, Fyodor A.; Yampolsky, Ilia V.; Sarkisyan, Karen S. published the artcile< Plants with genetically encoded autoluminescence>, Product Details of C11H8N2O3S2, the main research area is Nicotiana leaf caffeic acid hispidin synthase signaling bioluminescence imaging.

Abstract: Autoluminescent plants engineered to express a bacterial bioluminescence gene cluster in plastids have not been widely adopted because of low light output. We engineered tobacco plants with a fungal bioluminescence system that converts caffeic acid (present in all plants) into luciferin and report self-sustained luminescence that is visible to the naked eye. Our findings could underpin development of a suite of imaging tools for plants.

Nature Biotechnology published new progress about Bioluminescent imaging. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Product Details of C11H8N2O3S2.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Zhang, Kaiyue; Li, Zongjin published the artcile< Molecular Imaging of Therapeutic Effect of Mesenchymal Stem Cell-Derived Exosomes for Hindlimb Ischemia Treatment>, Safety of (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is exosome mesenchymal stem cell mol imaging hindlimb ischemia; Bioluminescent imaging; Exosome; Firefly luciferase; Hindlimb ischemia; Luciferin; MSC.

Critical limb ischemia is a major cause of morbidity and mortality worldwide. Recently, many studies confirmed that MSC-derived exosomes (MSC-exosomes) had potential therapeutic effect to treat hindlimb ischemia through pro-angiogenesis. The therapeutic angiogenesis is a critical measurement to judge the beneficial effect of MSC-exosomes treatment. Formerly, the therapeutic effect of MSC-exosomes was usually evaluated through clin. assessment and histopathol. examination Here, we describe a strategy to evaluate the therapeutic effect of MSC-exosomes by monitoring the therapeutic angiogenesis with bioluminescent imaging in hindlimb ischemia mice models.

Methods in Molecular Biology (New York, NY, United States) published new progress about Angiogenesis. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Safety of (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Evans, Bridget L.; Hurlstone, Adam F. L.; Clayton, Peter E.; Stevens, Adam; Shiels, Holly A. published the artcile< Glucose uptake as an alternative to oxygen uptake for assessing metabolic rate in Danio rerio larvae>, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is glucose uptake metabolic rate Danio rerio larvae; Cardiorespiratory; Development; Glucose; Metabolism; Method comparison; Respirometry; Zebrafish larvae.

Respirometry, based on oxygen uptake, is commonly employed for measuring metabolic rate. There is a growing need for metabolic rate measurements suitable for developmental studies, particularly in Danio rerio, where many important developmental stages occur at < 4 mm. However, respirometry becomes more challenging as the size of the organism reduces. Addnl., respirometry can be costly and require significant experience and tech. knowledge which may prohibit uptake in non-specialist/non-physiol. laboratories Thus, using equipment routine in most developmental/mol. biol. laboratories, we measured glucose uptake in 96-h post fertilization (hpf) zebrafish larvae and compared it to stop-flow respirometry measures of oxygen uptake to test whether glucose uptake was a suitable alternative measure of metabolic rate. A Passing-Bablok regression revealed that within a 95% limit of agreement, the rate of glucose uptake and the rate of oxygen uptake were equivalent as measures of metabolic rate in 96 hpf Danio rerio larvae. Thus, the methodol. we outline here may be a useful alternative or a complementary method for assessing metabolic rate in small organisms. Current Research in Physiology published new progress about Danio rerio. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Fretaud, Maxence; Descamps, Delphyne; Laubreton, Daphne; Rameix-Welti, Marie-Anne; Eleouet, Jean-Francois; Larcher, Thibaut; Galloux, Marie; Langevin, Christelle published the artcile< New look at RSV infection: tissue clearing and 3D imaging of the entire mouse lung at cellular resolution>, Product Details of C11H8N2O3S2, the main research area is mouse lung RSV infection cellular resolution tissue clearing imaging; 3D imaging of lungs; RSV infection; RSV tropism; inclusion bodies; tissue clearing; viral pathophysiology.

Respiratory Syncytial Virus (RSV) is the major cause of severe acute respiratory tract illness in young children worldwide and a main pathogen for the elderly and immune-compromised people. In the absence of vaccines or effective treatments, a better characterization of the pathogenesis of RSV infection is required. To date, the pathophysiol. of the disease and its diagnosis has mostly relied on chest X-ray and genome detection in nasopharyngeal swabs. The development of new imaging approaches is instrumental to further the description of RSV spread, virus-host interactions and related acute respiratory disease, at the level of the entire lung. By combining tissue clearing, 3D microscopy and image processing, we developed a novel visualization tool of RSV infection in undissected mouse lungs. Whole tissue anal. allowed the identification of infected cell subtypes, based on both morphol. traits and position within the cellular network. Furthermore, 3D imaging was also valuable to detect the cytoplasmic viral factories, also called inclusion bodies, a hallmark of RSV infection. Whole lung clearing and 3D deep imaging represents an unprecedented visualization method of infected lungs to allow insight into RSV pathophysiol. and improve the 2D histol. analyses.

Viruses published new progress about Acute respiratory disease. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Product Details of C11H8N2O3S2.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Su, Yichi; Walker, Joel R.; Park, Yunhee; Smith, Thomas P.; Liu, Lan Xiang; Hall, Mary P.; Labanieh, Louai; Hurst, Robin; Wang, David C.; Encell, Lance P.; Kim, Namdoo; Zhang, Feijie; Kay, Mark A.; Casey, Kerriann M.; Majzner, Robbie G.; Cochran, Jennifer R.; Mackall, Crystal L.; Kirkland, Thomas A.; Lin, Michael Z. published the artcile< Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals>, HPLC of Formula: 2591-17-5, the main research area is hydrofurimazine furimazine bioluminescence tumor.

Sensitive detection of two biol. events in vivo has long been a goal in bioluminescence imaging. Antares, a fusion of the luciferase NanoLuc to the orange fluorescent protein CyOFP, has emerged as a bright bioluminescent reporter with orthogonal substrate specificity to firefly luciferase (FLuc) and its derivatives such as AkaLuc. However, the brightness of Antares in mice is limited by the poor solubility and bioavailability of the NanoLuc substrate furimazine. Here, we report a new substrate, hydrofurimazine, whose enhanced aqueous solubility allows delivery of higher doses to mice. In the liver, Antares with hydrofurimazine exhibited similar brightness to AkaLuc with its substrate AkaLumine. Further chem. exploration generated a second substrate, fluorofurimazine, with even higher brightness in vivo. We used Antares with fluorofurimazine to track tumor size and AkaLuc with AkaLumine to visualize CAR-T cells within the same mice, demonstrating the ability to perform two-population imaging with these two luciferase systems.

Nature Methods published new progress about Animal gene Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (albumin-Cr). 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, HPLC of Formula: 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Othman, Ali; Katz, Evgeny; Smutok, Oleh published the artcile< Electrochemically switchable and tunable luciferase bioluminescence>, Safety of (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is luciferase bioluminescence biocatalyst porosity; Bioluminescence; Electrochemically switchable; Electrochemically tunable; Luciferase; Modified electrode.

The biocatalytic activity of electrode-immobilized luciferase followed by bioluminescence emitted from the electrode surface was reversibly tuned and switched by applying electrochem. signals. When a reductive potential (-0.9 V vs. Ag/AgCl) was applied, O2 was consumed at the electrode resulting in its depletion in a thin film near the electrode surface. This resulted in the inhibition of the immobilized luciferase which needs O2 for the biocatalytic reaction. Releasing the potential resulted in diffusional equilibration of the O2 local concentration with the bulk solution, then reactivating luciferase. Reversible inhibition-activation of luciferase was obtained upon cyclic application and releasing of the potential, resp.

Bioelectrochemistry published new progress about Bioluminescence. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Safety of (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Puhakka, Emmi; Santala, Ville published the artcile< Method for acrylic acid monomer detection with recombinant biosensor cells for enhanced plastic degradation monitoring from water environments>, Application In Synthesis of 2591-17-5, the main research area is acrylic acid monomer detection plastic degradation; Acrylic acid; Biosensor cells; Monomer detection; Plastic degradation; Poly(acrylic acid); Water environments.

Plastic debris degrades in the water environments due to various factors such as mech. stress. Small-sized degradation products, including plastic monomers, are currently monitored using equipment which might be unsuitable for screening. Here, we developed a recombinant whole-cell bacterial biosensor, which could be used for this type of monitoring. The Escherichia coli pBAV1K-ACU-lucFF cells contain a luciferase-based reporter system under the control of acrylic acid specific promoter. The biosensor cells were used to detect acrylic acid monomers from both sterile water and spiked lake water samples, indicating usability with environmental samples. Furthermore, poly(acrylic acid) was incubated in salt water, and the biosensor cells could identify acrylic acid monomers originating from it. Thus, the cells could be used to observe similar processes in the environment. The results show that the bacterial biosensors could complement the current research methods of plastic monomer monitoring in water environments with a potential for higher throughputs.

Marine Pollution Bulletin published new progress about Actinomyces kanamycini. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Application In Synthesis of 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica