Category: 2591-17-5

Chen, Shengyu; Zhao, Jingjin; Yang, Xing; Zhao, Shulin; Liu, Yi-Ming published the artcile< A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection>, Synthetic Route of 2591-17-5, the main research area is ATP microchip electrophoresis laser induced fluorescence.

An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells are at 30-150, 30-140, and 19-120 fmol per cell, resp.

Chemical Communications (Cambridge, United Kingdom) published new progress about Capillary electrophoresis (microchip). 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Synthetic Route of 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Carvalho, Mariele C.; Tomazini, Atilio; Prado, Rogilene A.; Viviani, Vadim R. published the artcile< Selective inhibition of Zophobas morio (Coleoptera: Tenebrionidae) luciferase-like enzyme luminescence by diclofenac and potential suitability for light-off biosensing>, Application In Synthesis of 2591-17-5, the main research area is diclofenac; luciferase-like enzyme; luminescence; toxicity.

The accumulation of toxic carboxylic compounds may cause severe effects on the environment and living organisms. A luciferase-like enzyme, previously cloned from the Malpighian tubules of the non-luminescent Zophobas morio mealworm, displays thioesterification activity with a wide range of carboxylic substrates, and produces weak red luminescence in the presence of ATP and firefly -luciferin, a xenobiotic for this organism. To better investigate the function of this enzyme in carboxylic xenobiotic detoxification, we analyzed the inhibitory effect of different xenobiotic carboxylic acids on the luminescence activity of this enzyme, including environmental pollutants and pharmaceutical compounds Noteworthy, the anti-inflammatory drug diclofenac severely inhibited this luciferase-like enzyme luminescence activity, both in in vitro (IC50 20 μM) and in vivo in bacterial cells assays, when compared with other beetle luciferases. Similar results were obtained with its brighter I327S mutant. Kinetic anal. of diclofenac′s effect on luminescence activity indicated mixed-type inhibition for both ATP and -luciferin. Modeling studies showed five potential binding sites for diclofenac, including the CoA binding site, which showed one of the highest binding constant Taken together, these results raise the possibility of using this luciferase-like enzyme for the development of novel whole-cell luminescent biosensors for diclofenac and similar drugs.

Luminescence published new progress about 2591-17-5. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Application In Synthesis of 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Ye, Sen; Hananya, Nir; Green, Ori; Chen, Hansen; Zhao, Angela Qian; Shen, Jiangang; Shabat, Doron; Yang, Dan published the artcile< A Highly Selective and Sensitive Chemiluminescent Probe for Real-Time Monitoring of Hydrogen Peroxide in Cells and Animals>, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is Chemiluminescent probe hydrogen peroxide bioimaging imaging agent; bioimaging; chemiluminescence; fluorescent probes; hydrogen peroxide; imaging agents.

Selective and sensitive mol. probes for hydrogen peroxide (H2O2), which plays diverse roles in oxidative stress and redox signaling, are urgently needed to investigate the physiol. and pathol. effects of H2O2. A lack of reliable tools for in vivo imaging has hampered the development of H2O2 mediated therapeutics. By combining a specific tandem Payne/Dakin reaction with a chemiluminescent scaffold, H2O2-CL-510 was developed as a highly selective and sensitive probe for detection of H2O2 both in vitro and in vivo. A rapid 430-fold enhancement of chemiluminescence was triggered directly by H2O2 without any laser excitation. Arsenic trioxide induced oxidative damage in leukemia was successfully detected. In particular, cerebral ischemia-reperfusion injury-induced H2O2 fluxes were visualized in rat brains using H2O2-CL-510, providing a new chem. tool for real-time monitoring of H2O2 dynamics in living animals.

Angewandte Chemie, International Edition published new progress about Biological imaging. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Chen, Liying; Chen, Zhi; Zheng, Shuang; Fan, Luhui; Zhu, Lixin; Yu, Jiandong; Tang, Chaoyuan; Liu, Qi; Xiong, Yang published the artcile< Study on mechanism of elemene reversing tumor multidrug resistance based on luminescence pharmacokinetics in tumor cells in vitro and in vivo>, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is elemene reversing tumor multidrug resistance luminescence pharmacokinetics.

While elemene (ELE) can reverse tumor multidrug resistance (MDR), the mechanisms for ELE reversing MDR remain unclear. Numerous studies have suggested that the efflux functionality of ATP-binding cassette (ABC) transporters, not their quantity, is more relevant to tumor MDR. However, no appropriate methods exist for real-time detection of the intracellular drug efflux caused by ABC transporters in vitro, especially in vivo, which hinders the examination of MDR reversal mechanisms. This study directly investigates the correlation between efflux functionality of ABC transporters and MDR reversal via ELE, using D-luciferin potassium salt (D-luc) as the chemotherapeutic substitute to study the intracellular drug efflux. Here, a luciferase reporter assay system combined with bioluminescence imaging confirmed that the efflux of D-luc from MCF-7/DOXFluc cells in vitro and in vivo was significantly reduced by ELE and when combined with Doxorubicin (DOX), ELE showed a synergistically anti-tumor effect in vitro and in vivo. Addnl., the luminescence pharmacokinetics of D-luc in MCF-7/DOXFluc cells and pharmacodynamics of the combined ELE and DOX in vivo showed a great correlation, implying that D-luc might be used as a probe to study ABC transporters-mediated efflux in order to explore mechanisms of traditional Chinese medicines reversing MDR.

RSC Advances published new progress about Bioluminescence. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Nakayama, Jun; Saito, Ryohei; Hayashi, Yusuke; Kitada, Nobuo; Tamaki, Shota; Han, Yuxuan; Semba, Kentaro; Maki, Shojiro A. published the artcile< High sensitivity in vivo imaging of cancer metastasis using a near-infrared luciferin analogue seMpai>, Application In Synthesis of 2591-17-5, the main research area is cancer metastasis IR luciferin analog sempai; in vivo imaging; luciferin analogue; metastasis; near-infrared bioluminescence.

Bioluminescence imaging (BLI) is useful to monitor cell movement and gene expression in live animals. However, D-luciferin has a short wavelength (560 nm) which is absorbed by tissues and the use of near-IR (NIR) luciferin analogs enable high sensitivity in vivo BLI. The AkaLumine-AkaLuc BLI system (Aka-BLI) can detect resolution at the single-cell level; however, it has a clear hepatic background signal. Here, to enable the highly sensitive detection of bioluminescence from the surrounding liver tissues, we focused on seMpai (C15H16N3O2S) which has been synthesized as a luciferin analog and has high luminescent abilities as same as AkaLumine. We demonstrated that seMpai BLI could detect micro-signals near the liver without any background signal. The solution of seMpai was neutral; therefore, seMpai imaging did not cause any adverse effect in mice. seMpai enabled a highly sensitive in vivo BLI as compared to previous techniques. Our findings suggest that the development of a novel mutated luciferase against seMpai may enable a highly sensitive BLI at the single-cell level without any background signal. Novel seMpai BLI system can be used for in vivo imaging in the fields of life sciences and medicine.

International Journal of Molecular Sciences published new progress about Bioluminescent imaging. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Application In Synthesis of 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Murty, Surya; Haile, Samuel T.; Beinat, Corinne; Aalipour, Amin; Alam, Israt S.; Murty, Tara; Shaffer, Travis M.; Patel, Chirag B.; Graves, Edward E.; Mackall, Crystal L.; Gambhir, Sanjiv S. published the artcile< Intravital imaging reveals synergistic effect of CAR T-cells and radiation therapy in a preclinical immunocompetent glioblastoma model>, HPLC of Formula: 2591-17-5, the main research area is disialoganglioside GD2 CAR T cell apoptosis glioblastoma; Chimeric antigen receptor (CAR); glioblastoma; imaging; immunotherapy; intravital microscopy.

Recent advances in novel immune strategies, particularly chimeric antigen receptor (CAR)-bearing T-cells, have shown limited efficacy against glioblastoma (GBM) in clin. trials. We currently have an incomplete understanding of how these emerging therapies integrate with the current standard of care, specifically radiation therapy (RT). Addnitionally, there is an insufficient number of preclin. studies monitoring these therapies with high spatiotemporal resolution To address these limitations, we report the first longitudinal fluorescence-based intravital microscopy imaging of CAR T-cells within an orthotopic GBM preclin. model to illustrate the necessity of RT for complete therapeutic response. Complete antitumor response in advanced syngeneic orthotopic models of GBM was achieved only when a single i.v. dose of GD2 CAR T-cells was following either sub-lethal whole-body irradiation or focal RT. Intravital microscopy imaging successfully visualized CAR T-cell homing and T-cell mediated apoptosis of tumor cells in real-time within the tumor stroma. Findings indicate that RT allows for rapid CAR T-cell extravasation from the vasculature and expansion within the tumor microenvironment. These exciting results highlight potential opportunities to improve IV adoptive T-cell administration in the treatment of GBM through concurrent RT. Addnl., they emphasize the need for advancements in immunotherapeutic homing to and extravasation through the tumor microenvironment.

OncoImmunology published new progress about Apoptosis. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, HPLC of Formula: 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Nakajima, Kanako; Hamada, Kazuko; Ito, Ryoga; Yoshida, Yukina; Sutherland, Kenneth; Ishikawa, Masayori; Ozaki, Michitaka; Shirato, Hiroki; Hamada, Toshiyuki published the artcile< Stability of -luciferin for bioluminescence to detect gene expression in freely moving mice for long durations>, Product Details of C11H8N2O3S2, the main research area is Dluciferin L luciferin antiobesity agent obesity cancer; Period1; circadian rhythm; in vivo imaging; luciferin.

Circadian disturbance of clock gene expression is a risk factor for diseases such as obesity, cancer, and sleep disorders. To study these diseases, it is necessary to monitor and analyze the expression rhythm of clock genes in the whole body for a long duration. The bioluminescent reporter enzyme firefly luciferase and its substrate -luciferin have been used to generate optical signals from tissues in vivo with high sensitivity. However, little information is known about the stability of -luciferin to detect gene expression in living animals for a long duration. In the present study, we examined the stability of a luciferin solution over 21 days. -Luciferin, which is synthesized using racemization of -luciferin, was at high concentrations after 21 days. In addition, we showed that bioluminescence of Period1 (Per1) expression in the liver was significantly decreased compared with the day 1 solution, although locomotor activity rhythm was not affected. These results showed that -luciferin should be applied to the mouse within, at most, 7 days to detect bioluminescence of Per1 gene expression rhythm in vivo.

Luminescence published new progress about Acyl-CoA-binding proteins Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Product Details of C11H8N2O3S2.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Shrestha, Tej B.; Basel, Matthew T. published the artcile< Using Naturally Occurring Bioluminescent Enzymes to Track Specific Cell Populations>, SDS of cas: 2591-17-5, the main research area is review natural bioluminescent enzyme cell population tracking; Bioluminescence; Luminol; Myeloperoxidase; NADPH oxidase; Neutrophil.

A review. Luminol-based bioluminescence imaging allows noninvasive tracking of oxidatively active cells such as neutrophils. Luminol is given i.v. or i.p., followed by bioluminescence imaging at 425 nm. Here we describe a method for tracking neutrophil extravasation into an inflammatory site, especially focusing on mammary carcinoma.

Methods in Molecular Biology (New York, NY, United States) published new progress about Bioluminescent imaging. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, SDS of cas: 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Chen, Tzu-Yin; Chen, Mei-Ru; Liu, Shan-Wen; Lin, Jin-Yan; Yang, Ya-Ting; Huang, Hsin-Ying; Chen, Jen-Kun; Yang, Chung-Shi; Lin, Kurt Ming-Chao published the artcile< Assessment of polyethylene glycol-coated gold nanoparticle toxicity and inflammation in vivo using NF-κB reporter mice>, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is polyethylene glycol coated gold nanoparticle toxicity inflammation reporter mice; NF-κB; PEG surface modification; gold nanoparticle; liver inflammation; reporter imaging; steatosis.

Polyethylene glycol (PEG) coating of gold nanoparticles (AuNPs) improves AuNP distribution via blood circulation. The use of PEG-coated AuNPs was shown to result in acute injuries to the liver, kidney, and spleen, but long-term toxicity has not been well studied. In this study, we investigated reporter induction for up to 90 days in NF-κB transgenic reporter mice following i.v. injection of PEG-coated AuNPs. The results of different doses (1 and 4μg AuNPs per g of body weight), particle sizes (13 nm and 30 nm), and PEG surfaces (methoxyl- or carboxymethyl-PEG 5 kDa) were compared. The data showed up to 7-fold NF-κB reporter induction in mouse liver from 3 h to 7 d post PEG-AuNP injection compared to saline-injected control mice, and gradual reduction to a level similar to control by 90 days. Agglomerates of PEG-AuNPs were detected in liver Kupffer cells, but neither gross pathol. abnormality in liver sections nor increased activity of liver enzymes were found at 90 days. Injection of PEG-AuNPs led to an increase in collagen in liver sections and elevated total serum cholesterol, although still within the normal range, suggesting that inflammation resulted in mild fibrosis and affected hepatic function. Administrating PEG-AuNPs inevitably results in nanoparticles entrapped in the liver; thus, further investigation is required to fully assess the long-term impacts by PEG-AuNPs on liver health.

International Journal of Molecular Sciences published new progress about Hepatotoxicity. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Ren, Yiqian; Qiang, Yao; Zhu, Beibei; Tang, Wei; Duan, Xinrui; Li, Zhengping published the artcile< General strategy for bioluminescence sensing of peptidase activity in vivo based on tumor-targeting probiotic>, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is human leucine aminopeptidase bioluminescence sensing tumor targeting probiotic mouse; dipeptidyl peptidase IV human bioluminescence sensing tumor targeting probiotic.

The abnormally expressed peptidases in human tissues are associated with many kinds of cancers. Monitoring of endogenous peptidase activity could allow us for pathophysiol. elucidation and early clin. diagnosis. Herein, we developed a general strategy for bioluminescence (BL) sensing of peptidase activity in vivo based on tumor-targeting probiotics. The probiotic that harbored a luciferase-encoding plasmid was used to target and colonize tumor and provide luciferase for BL imaging. The peptide-based probes Lc and GPc were applied to track leucine aminopeptidase (LAP) and dipeptidyl peptidase IV (DPPIV) activity, resp., by simply adding L-leucine and Gly-Pro dipeptides at the N-terminus of D-cysteine, which were specifically controlled by peptidase cleavage and released free D-cysteine to conduct a subsequent click condensation reaction with 2-cyano-6-hydroxybenzothiazole (HCBT) to produce firefly luciferin in situ, giving rise to a strong BL signal. Neither gene modification of cells of interest nor complicated synthesis was required in this BL system. Encouraged by these advantages, we successfully used our probes to monitor LAP and DPPIV activities in vitro and in vivo, resp. A good linearity between BL and peptidase was obtained in the concentration range of 2.5-40.0 mU/mL with a limit of detection (LOD) of 1.1 mU/mL (55 ng/mL) for LAP and 2.0-40.0 mU/mL with a LOD of 0.78 mU/mL (1.15 ng/mL) for DPPIV, resp. Addnl., approx. 5-fold (LAP) and 10-fold (DPPIV) differences in the BL signal before and after treatment with a specific inhibitor were also obtained for in vivo BL imaging. All these results reflected the potential application value of our probes in BL sensing of peptidase activity. We envision that our strategy may be a useful approach for monitoring a wide range of peptidases in tumors, especially in primary tumors.

Analytical Chemistry (Washington, DC, United States) published new progress about Animal tissue (human). 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica