Tag: M.W=250-300

Inouye, Satoshi; Sahara-Miura, Yuiko; Nakamura, Mitsuhiro; Hosoya, Takamitsu published the artcile< Expression, purification, and characterization of recombinant apoPholasin>, COA of Formula: C11H8N2O3S2, the main research area is apoPholasin glutathione transferase coelenterazine reactive oxygen species oxidation; Coelenteramide; Coelenteramine; Dehydrocoelenterazine; Photoproteins; Reactive oxygen.

Pholasin is a reactive oxygen-sensitive photoprotein that consists of an apoprotein (apoPholasin) and an unknown chromophore. The preferred human codon-optimized apoPholasin gene was transiently expressed in mammalian cells and apoPholasin was detected using an anti-recombinant apoPholasin antibody. For the first time, we found that apoPholasin secreted into the culture medium could catalyze the oxidation of coelenterazine (CTZ, a luciferin) to produce continuous luminescence. The fusion protein of apoPholasin and glutathione S-transferase (GST-apoPholasin) was successfully expressed as a soluble form in bacterial cells using the cold induction system. The purified GST-apoPholasin also had luminescence activity with CTZ, showing the bioluminescence emission peak at 461 nm, and the resultant product showed purple blue fluorescence under 365 nm light. Unexpectedly, the main oxidation product of CTZ was identified as coelenteramine (CTM), not coelenteramide (CTMD).

Protein Expression and Purificationpublished new progress about Absorption. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, COA of Formula: C11H8N2O3S2.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Dammann, Allison N.; Chamby, Anna B.; Catomeris, Andrew J.; Davidson, Kyle M.; Tettelin, Herve; van Pijkeren, Jan-Peter; Gopalakrishna, Kathyayini P.; Keith, Mary F.; Elder, Jordan L.; Ratner, Adam J.; Hooven, Thomas A. published the artcile< Genome-Wide fitness analysis of group B Streptococcus in human amniotic fluid reveals a transcription factor that controls multiple virulence traits>, Product Details of C11H8N2O3S2, the main research area is genome fitness analysis Streptococcus human amniotic fluid transcription factor; virulence trait.

Streptococcus agalactiae (group B Streptococcus; GBS) remains a dominant cause of serious neonatal infections. One aspect of GBS that renders it particularly virulent during the perinatal period is its ability to invade the chorioamniotic membranes and persist in amniotic fluid, which is nutritionally deplete and rich in fetal immunol. factors such as antimicrobial peptides. We used next-generation sequencing of transposon-genome junctions (Tn-seq) to identify five GBS genes that promote survival in the presence of human amniotic fluid. We confirmed our Tn-seq findings using a novel CRISPR inhibition (CRISPRi) gene expression knockdown system. This anal. showed that one gene, which encodes a GntR-class transcription factor that we named MrvR, conferred a significant fitness benefit to GBS in amniotic fluid. We generated an isogenic targeted deletion of the mrvR gene, which had a growth defect in amniotic fluid relative to the wild type parent strain. The mrvR deletion strain also showed a significant biofilm defect in vitro. Subsequent in vivo studies showed that while the mutant was able to cause persistent murine vaginal colonization, pregnant mice colonized with the mrvR deletion strain did not develop preterm labor despite consistent GBS invasion of the uterus and the fetoplacental units. In contrast, pregnant mice colonized with wild type GBS consistently deliver prematurely. In a sepsis model the mrvR deletion strain showed significantly decreased lethality. In order to better understand the mechanism by which this newly identified transcription factor controls GBS virulence, we performed RNA-seq on wild type and mrvR deletion GBS strains, which revealed that the transcription factor affects expression of a wide range of genes across the GBS chromosome. Nucleotide biosynthesis and salvage pathways were highly represented among the set of differentially expressed genes, suggesting that MrvR may be involved in regulating nucleotide availability.

PLoS Pathogenspublished new progress about Amniotic fluid. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Product Details of C11H8N2O3S2.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Liu, Jingjing; Tian, Zhenwei; Liu, Tianzhou; Wen, Dacheng; Ma, Zhiming; Liu, Yuanda; Zhu, Jiaming published the artcile< CHSY1 is upregulated and acts as tumor promotor in gastric cancer through regulating cell proliferation, apoptosis, and migration>, HPLC of Formula: 2591-17-5, the main research area is prognosis CHSY1 XIAP cell proliferation migration metastatic gastric cancer; CHSY1; Gastric cancer; cell apoptosis; cell migration; cell proliferation.

Gastric cancer is one of the most frequently diagnosed malignant tumors, with rapid progression and poor prognosis. The role of chondroitin sulfate synthase 1 (CHSY1) in the development and progression of gastric cancer was explored and clarified in this study. The immunohistochem. anal. of clin. tissue samples as well as data mining of public database showed that CHSY1 was significantly upregulated in gastric cancer and associated with more advanced tumor stage and poorer prognosis. In vitro loss-of-function experiments demonstrated the inhibited cell proliferation, colony formation, cell migration, as well as the promoted cell apoptosis by CHSY1 knockdown. Moreover, recovery of CHSY1 expression could attenuate the regulatory effects induced by CHSY1 knockdown. Correspondingly, gastric cancer cells with CHSY1 knockdown showed reduced tumorigenicity and slower tumor growth in vivo. In conclusion, this study identified CHSY1 as a tumor promotor in gastric cancer, which may be utilized as a novel indicator of patients′ prognosis and therapeutic target for developing more effective drug for GC treatment.

Cell Cyclepublished new progress about Apoptosis. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, HPLC of Formula: 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Martin-Burgos, Blanca; Wang, Wanqi; William, Ivana; Tir, Selma; Mohammad, Innus; Javed, Reja; Smith, Stormi; Cui, Yilin; Arzavala, Jessica; Mora, Dalilah; Smith, Ciearra B.; van der Vinne, Vincent; Molyneux, Penny C.; Miller, Stephen C.; Weaver, David R.; Leise, Tanya L.; Harrington, Mary E. published the artcile< Methods for detecting PER2:LUCIFERASE bioluminescence rhythms in freely moving mice>, Quality Control of 2591-17-5, the main research area is circadian rhythm bioluminescence gene expression photomultiplier tube; CycLuc1; PERIOD2; bioluminescence; circadian; in vivo; luciferase; peripheral oscillators; reporter gene.

Circadian rhythms are driven by daily oscillations of gene expression. An important tool for studying cellular and tissue circadian rhythms is the use of a gene reporter, such as bioluminescence from the reporter gene luciferase controlled by a rhythmically expressed gene of interest. Here we describe methods that allow measurement of circadian bioluminescence from a freely moving mouse housed in a standard cage. Using a LumiCycle In Vivo (Actimetrics), we determined conditions that allow detection of circadian rhythms of bioluminescence from the PER2 reporter, PER2::LUC, in freely behaving mice. The LumiCycle In Vivo applies a background subtraction that corrects for effects of room temperature on photomultiplier tube (PMT) output. We tested delivery of d-luciferin via a s.c. minipump and in the drinking water. We demonstrate spikes in bioluminescence associated with drinking bouts. Further, we demonstrate that a synthetic luciferase substrate, CycLuc1, can support circadian rhythms of bioluminescence, even when delivered at a lower concentration than d-luciferin, and can support longer-term studies. A small difference in phase of the PER2::LUC bioluminescence rhythms, with females phase leading males, can be detected with this technique. We share our anal. scripts and suggestions for further improvements in this method. This approach will be straightforward to apply to mice with tissue-specific reporters, allowing insights into responses of specific peripheral clocks to perturbations such as environmental or pharmacol. manipulations.

Journal of Biological Rhythmspublished new progress about Biological oscillations. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Quality Control of 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Yevtodiyenko, Aleksey; Bazhin, Arkadiy; Khodakivskyi, Pavlo; Godinat, Aurelien; Budin, Ghyslain; Maric, Tamara; Pietramaggiori, Giorgio; Scherer, Sandra S.; Kunchulia, Marina; Eppeldauer, George; Polyakov, Sergey V.; Francis, Kevin P.; Bryan, Jeffrey N.; Goun, Elena A. published the artcile< Portable bioluminescent platform for in vivo monitoring of biological processes in non-transgenic animals>, Name: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is bioluminescent transgenic animal biol process invivo monitoring.

Bioluminescent imaging (BLI) is one of the most powerful and widely used preclin. imaging modalities. However, the current technol. relies on the use of transgenic luciferase-expressing cells and animals and therefore can only be applied to a limited number of existing animal models of human disease. Here, we report the development of a “”portable bioluminescent”” (PBL) technol. that overcomes most of the major limitations of traditional BLI. We demonstrate that the PBL method is capable of noninvasive measuring the activity of both extracellular (e.g., dipeptidyl peptidase 4) and intracellular (e.g., cytochrome P 450) enzymes in vivo in non-luciferase-expressing mice. Moreover, we successfully utilize PBL technol. in dogs and human cadaver, paving the way for the translation of functional BLI to the noninvasive quantification of biol. processes in large animals. The PBL methodol. can be easily adapted for the noninvasive monitoring of a plethora of diseases across multiple species.

Nature Communicationspublished new progress about Animal gene, CYP3A Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Name: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Pena, Stella; Scarone, Laura; Manta, Eduardo; Stewart, Lindsay; Yardley, Vanessa; Croft, Simon; Serra, Gloria published the artcile< Synthesis of a Microcystis aeruginosa predicted metabolite with antimalarial activity>, Application In Synthesis of 96929-05-4, the main research area is peptidomimetic synthesis Microcystis aeruginosa metabolite antimalarial agent; peptide coupling oxazole thiazole macrocyclization.

The synthesis of a Microcystis aeruginosa predicted metabolite analog of aerucyclamide B was performed. This hexacyclopeptide was obtained from three heterocyclic building blocks by a convergent macrocycle-assembly methodol. The compound exhibited good in vitro antiplasmodial activity (IC50: 0.18μM, K1, chloroquine-resistant strain).

Bioorganic & Medicinal Chemistry Letterspublished new progress about Antimalarials. 96929-05-4 belongs to class thiazole, and the molecular formula is C12H18N2O4S, Application In Synthesis of 96929-05-4.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica