Tag: M.W=250-300

Healy, Alan R.; Wernke, Kevin M.; Kim, Chung Sub; Lees, Nicholas R.; Crawford, Jason M.; Herzon, Seth B. published the artcile< Synthesis and reactivity of precolibactin 886>, COA of Formula: C12H18N2O4S, the main research area is synthesis precolibactin 886 nucleophilic cleavage.

The clb gene cluster encodes the biosynthesis of metabolites known as precolibactins and colibactins. The clb pathway is found in gut commensal Escherichia coli, and clb metabolites are thought to initiate colorectal cancer via DNA crosslinking. Here we report confirmation of the structural assignment of the complex clb product precolibactin 886 via a biomimetic synthetic pathway. We show that an α-ketoimine linear precursor undergoes spontaneous cyclization to precolibactin 886 on HPLC purification Studies of this α-ketoimine and the related α-dicarbonyl revealed that these compounds are unexpectedly susceptible to nucleophilic cleavage under mildly basic conditions. This cleavage pathway forms other known clb metabolites or biosynthetic intermediates and explains the difficulties in isolating fully mature biosynthetic products. This cleavage also accounts for a recently identified colibactin-adenine adduct. The colibactin peptidase ClbP deacylates synthetic precolibactin 886 to form a non-genotoxic pyridone, which suggests precolibactin 886 lies off the path of the major biosynthetic route.

Nature Chemistry published new progress about Biomimetic synthesis. 96929-05-4 belongs to class thiazole, and the molecular formula is C12H18N2O4S, COA of Formula: C12H18N2O4S.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Zhang, Kaiyue; Li, Zongjin published the artcile< Molecular Imaging of Therapeutic Effect of Mesenchymal Stem Cell-Derived Exosomes for Hindlimb Ischemia Treatment>, Safety of (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is exosome mesenchymal stem cell mol imaging hindlimb ischemia; Bioluminescent imaging; Exosome; Firefly luciferase; Hindlimb ischemia; Luciferin; MSC.

Critical limb ischemia is a major cause of morbidity and mortality worldwide. Recently, many studies confirmed that MSC-derived exosomes (MSC-exosomes) had potential therapeutic effect to treat hindlimb ischemia through pro-angiogenesis. The therapeutic angiogenesis is a critical measurement to judge the beneficial effect of MSC-exosomes treatment. Formerly, the therapeutic effect of MSC-exosomes was usually evaluated through clin. assessment and histopathol. examination Here, we describe a strategy to evaluate the therapeutic effect of MSC-exosomes by monitoring the therapeutic angiogenesis with bioluminescent imaging in hindlimb ischemia mice models.

Methods in Molecular Biology (New York, NY, United States) published new progress about Angiogenesis. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Safety of (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Evans, Bridget L.; Hurlstone, Adam F. L.; Clayton, Peter E.; Stevens, Adam; Shiels, Holly A. published the artcile< Glucose uptake as an alternative to oxygen uptake for assessing metabolic rate in Danio rerio larvae>, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is glucose uptake metabolic rate Danio rerio larvae; Cardiorespiratory; Development; Glucose; Metabolism; Method comparison; Respirometry; Zebrafish larvae.

Respirometry, based on oxygen uptake, is commonly employed for measuring metabolic rate. There is a growing need for metabolic rate measurements suitable for developmental studies, particularly in Danio rerio, where many important developmental stages occur at < 4 mm. However, respirometry becomes more challenging as the size of the organism reduces. Addnl., respirometry can be costly and require significant experience and tech. knowledge which may prohibit uptake in non-specialist/non-physiol. laboratories Thus, using equipment routine in most developmental/mol. biol. laboratories, we measured glucose uptake in 96-h post fertilization (hpf) zebrafish larvae and compared it to stop-flow respirometry measures of oxygen uptake to test whether glucose uptake was a suitable alternative measure of metabolic rate. A Passing-Bablok regression revealed that within a 95% limit of agreement, the rate of glucose uptake and the rate of oxygen uptake were equivalent as measures of metabolic rate in 96 hpf Danio rerio larvae. Thus, the methodol. we outline here may be a useful alternative or a complementary method for assessing metabolic rate in small organisms. Current Research in Physiology published new progress about Danio rerio. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Fretaud, Maxence; Descamps, Delphyne; Laubreton, Daphne; Rameix-Welti, Marie-Anne; Eleouet, Jean-Francois; Larcher, Thibaut; Galloux, Marie; Langevin, Christelle published the artcile< New look at RSV infection: tissue clearing and 3D imaging of the entire mouse lung at cellular resolution>, Product Details of C11H8N2O3S2, the main research area is mouse lung RSV infection cellular resolution tissue clearing imaging; 3D imaging of lungs; RSV infection; RSV tropism; inclusion bodies; tissue clearing; viral pathophysiology.

Respiratory Syncytial Virus (RSV) is the major cause of severe acute respiratory tract illness in young children worldwide and a main pathogen for the elderly and immune-compromised people. In the absence of vaccines or effective treatments, a better characterization of the pathogenesis of RSV infection is required. To date, the pathophysiol. of the disease and its diagnosis has mostly relied on chest X-ray and genome detection in nasopharyngeal swabs. The development of new imaging approaches is instrumental to further the description of RSV spread, virus-host interactions and related acute respiratory disease, at the level of the entire lung. By combining tissue clearing, 3D microscopy and image processing, we developed a novel visualization tool of RSV infection in undissected mouse lungs. Whole tissue anal. allowed the identification of infected cell subtypes, based on both morphol. traits and position within the cellular network. Furthermore, 3D imaging was also valuable to detect the cytoplasmic viral factories, also called inclusion bodies, a hallmark of RSV infection. Whole lung clearing and 3D deep imaging represents an unprecedented visualization method of infected lungs to allow insight into RSV pathophysiol. and improve the 2D histol. analyses.

Viruses published new progress about Acute respiratory disease. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Product Details of C11H8N2O3S2.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Thompson, Robert E.; Jolliffe, Katrina A.; Payne, Richard J. published the artcile< Total Synthesis of Microcin B17 via a Fragment Condensation Approach>, Name: Ethyl 2-(((tert-butoxycarbonyl)amino)methyl)thiazole-4-carboxylate, the main research area is microcin B17 total synthesis peptide fragment coupling.

The total synthesis of the 43 amino acid-containing antibacterial peptide Microcin B17 (MccB17) is described. The natural product was synthesized via a convergent approach from a heterocycle-derived peptide and peptide thioester fragments prepared via Fmoc-strategy solid-phase peptide synthesis (SPPS). Final assembly was achieved in an efficient manner using two Ag(I)-assisted peptide ligation reactions to afford MccB17 in excellent overall yield.

Organic Letters published new progress about Amino acids, N-[(fluorenylmethoxy)carbonyl] Role: RCT (Reactant), RACT (Reactant or Reagent). 96929-05-4 belongs to class thiazole, and the molecular formula is C12H18N2O4S, Name: Ethyl 2-(((tert-butoxycarbonyl)amino)methyl)thiazole-4-carboxylate.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Su, Yichi; Walker, Joel R.; Park, Yunhee; Smith, Thomas P.; Liu, Lan Xiang; Hall, Mary P.; Labanieh, Louai; Hurst, Robin; Wang, David C.; Encell, Lance P.; Kim, Namdoo; Zhang, Feijie; Kay, Mark A.; Casey, Kerriann M.; Majzner, Robbie G.; Cochran, Jennifer R.; Mackall, Crystal L.; Kirkland, Thomas A.; Lin, Michael Z. published the artcile< Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals>, HPLC of Formula: 2591-17-5, the main research area is hydrofurimazine furimazine bioluminescence tumor.

Sensitive detection of two biol. events in vivo has long been a goal in bioluminescence imaging. Antares, a fusion of the luciferase NanoLuc to the orange fluorescent protein CyOFP, has emerged as a bright bioluminescent reporter with orthogonal substrate specificity to firefly luciferase (FLuc) and its derivatives such as AkaLuc. However, the brightness of Antares in mice is limited by the poor solubility and bioavailability of the NanoLuc substrate furimazine. Here, we report a new substrate, hydrofurimazine, whose enhanced aqueous solubility allows delivery of higher doses to mice. In the liver, Antares with hydrofurimazine exhibited similar brightness to AkaLuc with its substrate AkaLumine. Further chem. exploration generated a second substrate, fluorofurimazine, with even higher brightness in vivo. We used Antares with fluorofurimazine to track tumor size and AkaLuc with AkaLumine to visualize CAR-T cells within the same mice, demonstrating the ability to perform two-population imaging with these two luciferase systems.

Nature Methods published new progress about Animal gene Role: BSU (Biological Study, Unclassified), BIOL (Biological Study) (albumin-Cr). 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, HPLC of Formula: 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Othman, Ali; Katz, Evgeny; Smutok, Oleh published the artcile< Electrochemically switchable and tunable luciferase bioluminescence>, Safety of (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is luciferase bioluminescence biocatalyst porosity; Bioluminescence; Electrochemically switchable; Electrochemically tunable; Luciferase; Modified electrode.

The biocatalytic activity of electrode-immobilized luciferase followed by bioluminescence emitted from the electrode surface was reversibly tuned and switched by applying electrochem. signals. When a reductive potential (-0.9 V vs. Ag/AgCl) was applied, O2 was consumed at the electrode resulting in its depletion in a thin film near the electrode surface. This resulted in the inhibition of the immobilized luciferase which needs O2 for the biocatalytic reaction. Releasing the potential resulted in diffusional equilibration of the O2 local concentration with the bulk solution, then reactivating luciferase. Reversible inhibition-activation of luciferase was obtained upon cyclic application and releasing of the potential, resp.

Bioelectrochemistry published new progress about Bioluminescence. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Safety of (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Puhakka, Emmi; Santala, Ville published the artcile< Method for acrylic acid monomer detection with recombinant biosensor cells for enhanced plastic degradation monitoring from water environments>, Application In Synthesis of 2591-17-5, the main research area is acrylic acid monomer detection plastic degradation; Acrylic acid; Biosensor cells; Monomer detection; Plastic degradation; Poly(acrylic acid); Water environments.

Plastic debris degrades in the water environments due to various factors such as mech. stress. Small-sized degradation products, including plastic monomers, are currently monitored using equipment which might be unsuitable for screening. Here, we developed a recombinant whole-cell bacterial biosensor, which could be used for this type of monitoring. The Escherichia coli pBAV1K-ACU-lucFF cells contain a luciferase-based reporter system under the control of acrylic acid specific promoter. The biosensor cells were used to detect acrylic acid monomers from both sterile water and spiked lake water samples, indicating usability with environmental samples. Furthermore, poly(acrylic acid) was incubated in salt water, and the biosensor cells could identify acrylic acid monomers originating from it. Thus, the cells could be used to observe similar processes in the environment. The results show that the bacterial biosensors could complement the current research methods of plastic monomer monitoring in water environments with a potential for higher throughputs.

Marine Pollution Bulletin published new progress about Actinomyces kanamycini. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Application In Synthesis of 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Chen, Shengyu; Zhao, Jingjin; Yang, Xing; Zhao, Shulin; Liu, Yi-Ming published the artcile< A novel intracellular signal amplification strategy for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection>, Synthetic Route of 2591-17-5, the main research area is ATP microchip electrophoresis laser induced fluorescence.

An intracellular signal amplification strategy was developed for the quantification of ATP in single cells by microchip electrophoresis with laser-induced fluorescence detection. By using the method proposed, intracellular ATP levels in single HeLa, HepG2 and HL-7702 cells are at 30-150, 30-140, and 19-120 fmol per cell, resp.

Chemical Communications (Cambridge, United Kingdom) published new progress about Capillary electrophoresis (microchip). 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Synthetic Route of 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Carvalho, Mariele C.; Tomazini, Atilio; Prado, Rogilene A.; Viviani, Vadim R. published the artcile< Selective inhibition of Zophobas morio (Coleoptera: Tenebrionidae) luciferase-like enzyme luminescence by diclofenac and potential suitability for light-off biosensing>, Application In Synthesis of 2591-17-5, the main research area is diclofenac; luciferase-like enzyme; luminescence; toxicity.

The accumulation of toxic carboxylic compounds may cause severe effects on the environment and living organisms. A luciferase-like enzyme, previously cloned from the Malpighian tubules of the non-luminescent Zophobas morio mealworm, displays thioesterification activity with a wide range of carboxylic substrates, and produces weak red luminescence in the presence of ATP and firefly -luciferin, a xenobiotic for this organism. To better investigate the function of this enzyme in carboxylic xenobiotic detoxification, we analyzed the inhibitory effect of different xenobiotic carboxylic acids on the luminescence activity of this enzyme, including environmental pollutants and pharmaceutical compounds Noteworthy, the anti-inflammatory drug diclofenac severely inhibited this luciferase-like enzyme luminescence activity, both in in vitro (IC50 20 μM) and in vivo in bacterial cells assays, when compared with other beetle luciferases. Similar results were obtained with its brighter I327S mutant. Kinetic anal. of diclofenac′s effect on luminescence activity indicated mixed-type inhibition for both ATP and -luciferin. Modeling studies showed five potential binding sites for diclofenac, including the CoA binding site, which showed one of the highest binding constant Taken together, these results raise the possibility of using this luciferase-like enzyme for the development of novel whole-cell luminescent biosensors for diclofenac and similar drugs.

Luminescence published new progress about 2591-17-5. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Application In Synthesis of 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica