Tag: M.W=250-300

Zheng, Sheng; Zhang, Ying; Chen, Shujie; Zhang, Zeyu; Chen, Fei; Zhang, Zizhen; Hu, Zhenhua; Tian, Jie; Wang, Liangjing published the artcile< A preliminary study of dual-band confocal laser endomicroscopy combined with image mosaic in the diagnosis of liver cancer>, Application In Synthesis of 2591-17-5, the main research area is diagnosis liver cancer confocal laser endomicroscopy; Confocal laser endomicroscopy; Fluorescein sodium; Image mosaic; Indocyanine green; Primary liver cancer; Tumor margin.

Accurate identification of tumor tissues and their margins are still challenging for conventional clin. imaging methods during liver cancer surgery. In this study, dual-band confocal laser endomicroscopy (CLE) combined with image mosaic was used to guide liver cancer surgery. In the experiments with mice bearing orthotropic liver tumor, CLE can accurately detect the tumors and identify their margins with two excitation wavelengths of 488 nm and 660 nm by clin. available dyes fluorescein sodium (FS) or indocyanine green (ICG). The mosaic CLE images enlarged the imaging field and detected the liver tumor margins more accurately. Normal liver tissues fluorescence intensity of CLE images was significantly higher than that of tumor tissues in the same tumor-bearing mice (P < 0.0001). Overall, dual-band CLE imaging demonstrates to be a promising method to identify liver tumor tissues and margins, which has the prospect of clin. application and helps to achieve intraoperative radical resection. Nanomedicine (New York, NY, United States) published new progress about Anesthesia. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Application In Synthesis of 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Novickij, Vitalij; Zinkeviciene, Aukse; Malysko, Veronika; Novickij, Jurij; Kulbacka, Julita; Rembialkowska, Nina; Girkontaite, Irute published the artcile< Bioluminescence as a sensitive electroporation indicator in sub-microsecond and microsecond range of electrical pulses>, HPLC of Formula: 2591-17-5, the main research area is bleomycin bioluminescence electroporation indicator elec pulse electrochemotherapy tumor permeabilization; Bioluminescence; Bleomycin; Electrochemotherapy; Permeabilization; Tumors.

The cell membrane permeabilization in electroporation studies is usually quantified using fluorescent markers such as propidium iodide (PI) or YO-PRO, while Chinese Hamster Ovary cell line frequently serves as a model. In this work, as an alternative, we propose a sensitive methodolgy for detection and anal. of electroporation phenomenon based on bioluminescence. Luminescent mice myeloma SP2/0 cells (transfected using Luciferase-pcDNA3 plasmid) were used as a cell model. Electroporation has been studied using the 0.1-5μs x 250 and 100μs x 1-8 pulsing protocols in 1-2.5 kV/cm PEF range. It was shown that the bioluminescence response is dependent on the cell permeabilization state and can be effectively used to detect even weak permeabilization. During saturated permeabilization the methodol. accurately predicts the losses of cell viability due to irreversible electroporation. The results have been superpositioned with permeabilization and pore resealing (1 h post-treatment) data using PI. Also, the viability of the cells was evaluated. Lastly, the SP2/0 tumors have been developed in BALB/C mice and the methodol. has been tested in vivo using electrochemotherapy with bleomycin.

Journal of Photochemistry and Photobiology, B: Biology published new progress about Bioluminescence. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, HPLC of Formula: 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Pena, S.; Fagundez, C.; Medeiros, A.; Comini, M.; Scarone, L.; Sellanes, D.; Manta, E.; Tulla-Puche, J.; Albericio, F.; Stewart, L.; Yardley, V.; Serra, G. published the artcile< Synthesis of cyclohexapeptides as antimalarial and anti-trypanosomal agents>, Related Products of 96929-05-4, the main research area is cyclohexa peptide synthesis antimalarial antitrypanosomal agent structure activity; solid phase peptide synthesis macrocyclization.

Cyclohexapeptide analogs of natural products were obtained in very good yields by a combination of solid-phase peptide synthesis, for the linear peptide, and solution cyclization. The activities against Plasmodium falciparum K1, Trypanosoma brucei brucei and murine macrophages (cell line J774) of these novel compounds and azolic macrocycles, previously reported by us, were evaluated. Seven macrocycles showed submicromolar activities against Plasmodium falciparum K1 and a high selectivity (SI > 125) for the parasite. In addition, two compounds displayed one digit micromolar EC50 against T. brucei brucei and satisfactory selectivity (SI 82 and 95). Preliminary structure-activity relationships are presented.

MedChemComm published new progress about Antimalarials. 96929-05-4 belongs to class thiazole, and the molecular formula is C12H18N2O4S, Related Products of 96929-05-4.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Billin, Andrew N.; Bantscheff, Marcus; Drewes, Gerard; Ghidelli-Disse, Sonja; Holt, Jason A.; Kramer, Henning F.; McDougal, Alan J.; Smalley, Terry L.; Wells, Carrow W.; Zuercher, William J.; Henke, Brad R. published the artcile< Discovery of Novel Small Molecules that Activate Satellite Cell Proliferation and Enhance Repair of Damaged Muscle>, Formula: C12H18N2O4S, the main research area is muscle regeneration.

Skeletal muscle progenitor stem cells (referred to as satellite cells) represent the primary pool of stem cells in adult skeletal muscle responsible for the generation of new skeletal muscle in response to injury. Satellite cells derived from aged muscle display a significant reduction in regenerative capacity to form functional muscle. This decrease in functional recovery has been attributed to a decrease in proliferative capacity of satellite cells. Hence, agents that enhance the proliferative abilities of satellite cells may hold promise as therapies for a variety of pathol. settings, including repair of injured muscle and age- or disease-associated muscle wasting. Through phenotypic screening of isolated murine satellite cells, we identified a series of 2,4-diaminopyrimidines (e.g., 2) that increased satellite cell proliferation. Importantly, compound 2 was effective in accelerating repair of damaged skeletal muscle in an in vivo mouse model of skeletal muscle injury. While these compounds were originally prepared as c-Jun N-terminal kinase 1 (JNK-1) inhibitors, structure-activity analyses indicated JNK-1 inhibition does not correlate with satellite cell activity. Screening against a broad panel of kinases did not result in identification of an obvious mol. target, so we conducted cell-based proteomics experiments in an attempt to identify the mol. target(s) responsible for the potentiation of the satellite cell proliferation. These data provide the foundation for future efforts to design improved small mols. as potential therapeutics for muscle repair and regeneration.

ACS Chemical Biology published new progress about Heterocyclic aromatic compounds Role: PAC (Pharmacological Activity), SPN (Synthetic Preparation), THU (Therapeutic Use), BIOL (Biological Study), PREP (Preparation), USES (Uses). 96929-05-4 belongs to class thiazole, and the molecular formula is C12H18N2O4S, Formula: C12H18N2O4S.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Fagundez, Catherine; Serra, Gloria published the artcile< Studies on synthesis of amino acid derived thiazoles. Preparation of bis-thiazoles as key fragments of aerucyclamide analogs>, Formula: C12H18N2O4S, the main research area is thiazole bisthiazole preparation.

The scope and limitations of Hantzsch, modified Hantzsch and Kelly methodologies for the synthesis of amino acid derived thiazoles are presented. In addition, the syntheses of bisthiazoles as key fragments of natural products and analogs are described. The Kelly’s methodol. followed by oxidation provides the desired N-Cbz protected thiazole after purification According with the authors’ results the Fmoc or Boc protecting groups are not compatible with the conditions used in this methodol. Modifications of the temperature and reagents used in the Hantzsch thiazole synthesis enabled the preparation of chiral thiazole building blocks without racemization and in good yields.

Heterocyclic Letters published new progress about 96929-05-4. 96929-05-4 belongs to class thiazole, and the molecular formula is C12H18N2O4S, Formula: C12H18N2O4S.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Wang, Yixuan; Chen, Quan; Wu, Di; Chen, Qifeng; Gong, Guanghui; He, Liuqing; Wu, Xiaoying published the artcile< Lamin-A interacting protein Hsp90 is required for DNA damage repair and chemoresistance of ovarian cancer cells>, Category: thiazole, the main research area is laminA protein Hsp DNA damage chemoresistance ovarian cancer cell.

Ovarian cancer is the most malignant gynecol. cancer. Previous studies found that lamin-A was associated with DNA damage repair proteins but the underlying mechanism remains unclear. We speculate that this may be related to its interacting proteins, such as Hsp90. The aim of this study is to investigate the effects of Hsp90 on DNA damage repair and chemoresistance of ovarian cancer cells. In our research, co-immunoprecipitation (co-IP) and mass spectrometry (MS) were used to identify proteins interacting with lamin-A and the interaction domain. Next, the relationship between lamin-A and Hsp90 was explored by Western blotting (WB) and immunofluorescence staining. Then, effect of Hsp90 inhibition on DNA damage repair was assessed through detecting Rad50 and Ku80 by WB. Furthermore, to test the roles of 17-AAG on cell chemosensitivity, CCK-8 and colony formation assay were carried out. Meanwhile, IC50 of cells were calculated, followed by immunofluorescence to detect DNA damage. At last, the mouse xenograft model was used in determining the capacity of 17-AAG and DDP to suppress tumor growth and metastatic potential. The results showed that lamin-A could interact with Hsp90 via the domain of lamin-A1-430. Besides, the distribution of Hsp90 could be affected by lamin-A. After lamin-A knockdown, Hsp90 decreased in the cytoplasm and increased in the nucleus, suggesting that the interaction between lamin-A and Hsp90 may be related to the nucleocytoplasmic transport of Hsp90. Moreover, inhibition of Hsp90 led to an obvious decrease in the expression of DSBs (DNA double-strand break) repair proteins, as well as cell proliferation ability upon DDP treatment and IC50 of DDP, causing more serious DNA damage. In addition, the combination of 17-AAG and DDP restrained the growth of ovarian cancer efficiently in vivo and prolonged the survival time of tumor-bearing mice.

Cell Death & Disease published new progress about Antitumor agents. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Category: thiazole.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Zambito, Giorgia; Gaspar, Natasa; Ridwan, Yanto; Hall, Mary P.; Shi, Ce; Kirkland, Thomas A.; Encell, Lance P.; Loewik, Clemens; Mezzanotte, Laura published the artcile< Evaluating Brightness and Spectral Properties of Click Beetle and Firefly Luciferases Using Luciferin Analogues: Identification of Preferred Pairings of Luciferase and Substrate for In Vivo Bioluminescence Imaging>, Name: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is beetle firefly luciferase dentification bioluminescence imaging; Bioluminescence; Emission spectrum; In vivo imaging; Luciferase; Luciferin.

Currently, a variety of red and green beetle luciferase variants are available for bioluminescence imaging (BLI). In addition, new luciferin analogs providing longer wavelength luminescence have been developed that show promise for improved deep tissue imaging. However, a detailed assessment of these analogs (e.g., Akalumine-HCl, CycLuc1, and amino naphthyl luciferin (NH2-NpLH2)) combined with state of the art luciferases has not been performed. The aim of this study was to evaluate for the first time the in vivo brightness and spectral characteristics of firefly (Luc2), click beetle green (CBG99), click beetle red 2 (CBR2), and Akaluc luciferases when paired with different D-luciferin (D-LH2) analogs in vivo. Transduced human embryonic kidney (HEK 293T) cells expressing individual luciferases were analyzed both in vitro and in mice (via s.c. injection). Following introduction of the luciferins to cells or animals, the resulting bioluminescence signal and photon emission spectrum were acquired using a sensitive charge-coupled device (CCD) camera equipped with a series of band pass filters and spectral unmixing software. Our in vivo anal. resulted in four primary findings: (1) the best substrate for Luc2, CBG99, and CBR2 in terms of signal strength was D-luciferin; (2) the spectra for Luc2 and CBR2 were shifted to a longer wavelength when Akalumine-HCl was the substrate; (3) CBR2 gave the brightest signal with the near-IR substrate, NH2-NpLH2; and (4) Akaluc was brighter when paired with either CycLuc1 or Akalumine-HCl when paired with D-LH2. We believe that the exptl. results described here should provide valuable guidance to end users for choosing the correct luciferin/luciferase pairs for a variety of BLI applications.

Molecular Imaging and Biology published new progress about Bioluminescence. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Name: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Godara, Amandeep; Zhou, Ping; Kugelmass, Adin; Ma, Xun; Rosenthal, Benjamin; Toskic, Denis; Fogaren, Teresa; Varga, Cindy; Comenzo, Raymond L. published the artcile< Presence of soluble and cell-surface B-cell maturation antigen in systemic light-chain amyloidosis and its modulation by gamma-secretase inhibition>, Quality Control of 2591-17-5, the main research area is light chain amyloidosis gamma secretase B cell maturation antigen.

This article describes about presence of soluble and cell-surface B-cell maturation antigen in systemic light-chain amyloidosis and its modulation by gammasecretase inhibition.

American Journal of Hematology published new progress about Amyloidosis (systemic light-chain). 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Quality Control of 2591-17-5.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Novickij, Vitalij; Zinkeviciene, Aukse; Radzeviciute, Eivina; Kulbacka, Julita; Rembialkowska, Nina; Novickij, Jurij; Girkontaite, Irute published the artcile< Bioluminescent calcium mediated detection of nanosecond electroporation: Grasping the differences between 100 ns and 100μs pulses>, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid, the main research area is bioluminescent calcium nanosecond electroporation; Bioluminescence; Calcium electroporation; Kinetic measurement; Membrane permeabilization; nsPEF.

Electroporation is a phenomenon of transient or irreversible permeabilization of the cell membrane after pulsed elec. field treatment. Fluorescent probes are frequently used to assess the extent of permeabilization, however, as an alternative, a D-luciferin oxidation-based method can be used. In this work, we have used sequences of a microsecond (1.3 kV/cm x 100μs) and nanosecond (12.5 kV/cm x 100 ns) pulses to trigger various levels of cell permeabilization and assessed the differences in the response using a conventional fluorescent probe (YO-PRO-1 (YP)) and D-luciferin oxidation methodol. The nanosecond pulses (n = 5-100) have been delivered with 1 kHz repetition frequency, and the results were compared with 1 MHz protocols. Addnl., the effects of extracellular Ca2+ have been assessed. Various concentrations of CaCl2 (2, 5, and 10 mM) have been used, and it was shown that the bioluminescence of the cells after electroporation depends on extracellular calcium concentration It was shown that the changes in bioluminescence signal could be used as a marker of cell membrane permeabilization on par with YP assay when calcium is added and thus, effectively employed for anal. of electroporation phenomenon in vitro both for nanosecond and microsecond pulses.

Bioelectrochemistry published new progress about Electroporation. 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, Recommanded Product: (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic acid.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica

Tiwari, Dhermendra K.; Tiwari, Manisha; Jin, Takashi published the artcile< Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution in vivo optical imaging>, COA of Formula: C11H8N2O3S2, the main research area is review NIR fluorescent proteins bioluminescent PET optical diagnostics.

A review. In the last few years, high-resolution near-IR (NIR) optical imaging has become an indispensable modality for non-invasive visualization of deep tissues both in fundamental life science and preclin. research. This is due to the high tissue permeability, low absorption and low scattering of NIR light as well as the low autofluorescence in the NIR wavelength region (700-1400 nm) in living systems. Compared to magnetic resonance imaging (MRI), X-ray computer tomog. (X-ray CT), and positron emission tomog. (PET), NIR optical imaging has a high spatiotemporal resolution (∼Μm) enough to visualize cellular dynamics at the whole-body level. Addnl., NIR optical imaging does not require high-energy ionizing radiation, such as X-rays, that leads to serious radiation damage of living cells. Furthermore, NIR optical imaging can easily achieve mol. imaging with the aid of NIR optical probes, which specifically bind to biomarkers expressed on cell surfaces. Thus, NIR optical imaging has great potential to be used for non-invasive optical diagnostics of diseases in medical and clin. fields. For such NIR optical imaging, NIR fluorescent probes with high brightness and biocompatibility are crucial. Although a variety of NIR imaging probes based on nanoparticles such as quantum dots and dye-incorporated polymers have been developed, possible applications of these imaging probes to optical contrast agents are limited due to their cytotoxicity. In contrast, fluorescent proteins and bioluminescence-based probes are highly biocompatible and practical for biomedical applications. During the last few years, a variety of NIR optical probes based on engineered proteins have been reported for fluorescence and/or bioluminescence in vivo imaging. This review describes the recent progress on NIR fluorescent proteins and bioluminescence-based probes for high-resolution in vivo optical imaging. The review also covers several cutting-edge optical imaging techniques using NIR fluorescent proteins and bioluminescent probes.

Materials Advances published new progress about Bacterial phytochrome BphP1 Role: BSU (Biological Study, Unclassified), BIOL (Biological Study). 2591-17-5 belongs to class thiazole, and the molecular formula is C11H8N2O3S2, COA of Formula: C11H8N2O3S2.

Referemce:
Thiazole | C3H3NS – PubChem,
Thiazole | chemical compound | Britannica